ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
Chloramphenicol 3-O-phosphotransferase (CPT) from Streptomyces venezuelae ISP5230, a novel chloramphenicol-inactivating kinase, has been overexpressed and purified using Escherichia coli as the heterologous host. Crystals of CPT in complex with its substrate chloramphenicol (Cm) were obtained which were suitable for X-ray diffraction. The crystals belong to the cubic space group I4132 with unit-cell dimension a = 200.0 Å. The initial CPT crystals diffracted to 3.5 Å and the diffraction was improved significantly upon adding acetonitrile and Cm to the crystallization drop. The CPT–Cm crystals diffract to at least 2.8 Å resolution.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S0907444999003029
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