Publication Date:
1988-01-15
Description:
The Texas male-sterile cytoplasm (cms-T) of maize carries the cytoplasmically inherited trait of male sterility. Mitochondria isolated from cms-T maize are specifically sensitive to a toxin (BmT-toxin) produced by the fungal pathogen Bipolaris maydis, race T, and the carbamate insecticide methomyl. A mitochondrial gene unique to cms-T maize, which produces a 13-kilodalton polypeptide associated with cytoplasmic male sterility, was expressed in Escherichia coli. After addition of BmT-toxin or methomyl, inhibition of whole cell respiration and swelling of spheroplasts were observed in Escherichia coli cultures producing the novel mitochondrial protein; these effects are similar to those observed with isolated cms-T mitochondria. The amino-terminal region of the 13-kilodalton polypeptide appears to be essential for proper interaction with the BmT-toxin and methomyl. These results implicate the 13-kilodalton polypeptide in conferring toxin sensitivity to mitochondria of cms-T maize.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Dewey, R E -- Siedow, J N -- Timothy, D H -- Levings, C S 3rd -- New York, N.Y. -- Science. 1988 Jan 15;239(4837):293-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Crop Science, North Carolina State University, Raleigh 27695.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3276005" target="_blank"〉PubMed〈/a〉
Keywords:
Bacterial Proteins/*pharmacology
;
Cloning, Molecular
;
Codon
;
Dicyclohexylcarbodiimide/pharmacology
;
Escherichia coli/drug effects/*genetics/metabolism
;
Methomyl/pharmacology
;
Mitochondria/*analysis
;
Molecular Weight
;
Mycotoxins/*pharmacology
;
Oxygen Consumption/drug effects
;
Plant Proteins/*genetics/physiology
;
Plants/analysis/drug effects/*genetics
;
Plasmids
;
Promoter Regions, Genetic
;
Zea mays
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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