Publication Date:
1991-10-25
Description:
SP-B is a protein in pulmonary surfactant that is, in greatest part, responsible for resistance to surface tension and prevention of collapse of pulmonary alveoli. Peptides of 21 residues, synthesized following the sequence of SP-B or resembling the hydrophobic and hydrophilic domains of SP-B (such as RLLLLRLLLLRLLLLRLLLLR, R, Arg, and L, Leu), enhanced the abilities of phospholipids to reduce surface tension both in vitro and in vivo. Intermittent positively charged residues were essential for this activity. The SP-B-like peptides were found by tryptophan fluorescence to partition within the phospholipid layer in contact with both polar head groups and acyl side chains. These data, together with findings that the SP-B-related peptides increase inter- and intramolecular order of the phospholipid layer, suggest that SP-B resists surface tension by increasing lateral stability of the phospholipid layer.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Cochrane, C G -- Revak, S D -- GM-37696/GM/NIGMS NIH HHS/ -- HL-23584/HL/NHLBI NIH HHS/ -- New York, N.Y. -- Science. 1991 Oct 25;254(5031):566-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Immunology, Scripps Research Institute, La Jolla, CA 92037.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1948032" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Kinetics
;
Molecular Sequence Data
;
Peptides/*chemical synthesis/chemistry
;
Phospholipids/metabolism
;
Proteolipids/chemistry/*metabolism
;
Pulmonary Surfactants/chemistry/*metabolism
;
Structure-Activity Relationship
;
Surface Tension
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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