Publication Date:
2007-01-16
Description:
The bacterial endosymbiont of the deep-sea tube worm Riftia pachyptila has never been successfully cultivated outside its host. In the absence of cultivation data, we have taken a proteomic approach based on the metagenome sequence to study the metabolism of this peculiar microorganism in detail. As one result, we found that three major sulfide oxidation proteins constitute approximately 12% of the total cytosolic proteome, which highlights the essential role of these enzymes for the symbiont's energy metabolism. Unexpectedly, the symbiont uses the reductive tricarboxylic acid cycle in addition to the previously identified Calvin cycle for CO2 fixation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Markert, Stephanie -- Arndt, Cordelia -- Felbeck, Horst -- Becher, Dorte -- Sievert, Stefan M -- Hugler, Michael -- Albrecht, Dirk -- Robidart, Julie -- Bench, Shellie -- Feldman, Robert A -- Hecker, Michael -- Schweder, Thomas -- New York, N.Y. -- Science. 2007 Jan 12;315(5809):247-50.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institute of Marine Biotechnology, Walther-Rathenau-Strasse 49, D-17489 Greifswald, Germany.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17218528" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Bacterial Proteins/analysis/*metabolism
;
Carbon/metabolism
;
Carbon Dioxide/metabolism
;
Chemoautotrophic Growth
;
Citric Acid Cycle
;
Cytosol/metabolism
;
*Ecosystem
;
Energy Metabolism
;
Gammaproteobacteria/enzymology/genetics/*metabolism
;
Genome, Bacterial
;
Hydrogen Sulfide/metabolism
;
Hydrogen-Ion Concentration
;
Metabolic Networks and Pathways
;
Molecular Sequence Data
;
Oxidation-Reduction
;
Pacific Ocean
;
Polychaeta/*microbiology
;
Proteome
;
*Proteomics
;
Sulfur/metabolism
;
*Symbiosis
;
Temperature
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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