ISSN:
1617-4623
Keywords:
Key words Initiator tRNA
;
2′-O-Ribosyl phosphate modification
;
RIT1 gene
;
Translation
;
Synergistic growth defects
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The Saccharomyces cerevisiae gene RIT1 encodes a phospho-ribosyl transferase that exclusively modifies the initiator tRNA (tRNAMet i) by the addition of a 2′-O-ribosyl phosphate group to Adenosine 64. As a result, tRNAMet i is prevented from participating in the elongation steps of protein synthesis. We previously showed that the modification is not essential for the function of tRNAMet i in the initiation of translation, since rit1 null strains are viable and show no obvious growth defects. Here, we demonstrate that yeast strains in which a rit1 null allele is combined with mutations in any of the genes for the three subunits of eukaryotic initiation factor-2 (eIF-2), or with disruption alleles of two of the four initiator methionine tRNA (IMT) genes, show synergistic growth defects. A multicopy plasmid carrying an IMT gene can alleviate these defects. On the other hand, introduction of a high-copy-number plasmid carrying the TEF2 gene, which encodes the eukaryotic elongation factor 1α (eEF-1α), into rit1 null strains with two intact IMT genes had the opposite effect, indicating that increased levels of eEF-1α are deleterious to these strains, presumably due to sequestration of the unmodified met-tRNAMet i for elongation. Thus, under conditions in which the components of the ternary met-tRNAMet i:GTP:eIF-2 complex become limiting or are functionally impaired, the presence of the 2′-O-ribosyl phosphate modification in tRNAMet i is important for the provision of adequate amounts of tRNAMet i for formation of this ternary complex.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004380051045
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