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  • 1
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    Phylogeny of bovine species based on AFLP fingerprinting (2002)
    Springer Nature
    Details
    Publication Date: 2002-01-01
    Print ISSN: 0018-067X
    Electronic ISSN: 1365-2540
    Topics: Biology
    Published by Springer Nature
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  • 2
    Electronic Resource
    Electronic Resource
    Mammalian species identification by interspersed repeat PCR fingerprinting (1998)
    Springer
    Journal of industrial microbiology and biotechnology 21 (1998), S. 121-127 
    Details
    ISSN: 1476-5535
    Keywords: Keywords: species identification; animal tissues; PCR; DNA fingerprinting
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Most DNA methods for species identification of animal tissues test the presence/absence of one species per assay, requiring several tests for a complete analysis and prior knowledge of the species that are potentially present in the sample. Here we demonstrate that PCR with fluorescently labeled MIR (mammalian-wide interspersed repeat) primers generate fingerprints that are suitable for rapid identification of known and unknown species on an automatic sequencing apparatus and with computer-assisted data processing. The method allows the analysis of processed meat samples and offers a convenient alternative to sequencing of mitochondrial DNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/sj.jim.2900540
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  • 3
    Electronic Resource
    Electronic Resource
    Species identification in meat by using PCR-generated satellite probes (1998)
    Springer
    Journal of industrial microbiology and biotechnology 21 (1998), S. 115-120 
    Details
    ISSN: 1476-5535
    Keywords: Keywords: species identification; DNA isolation; meat testing; satellite DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A convenient DNA-based identification system is described for testing the species origin of meat samples. Probes are generated by PCR with primers binding to species-specific satellite DNA and hybridized to DNA purified from meat. This method is more robust and versatile than methods based on oligonucleotide hybridization. With the exception of a slight cross-reaction of mutton and beef, each probe only recognized the species from which it was derived. Purifying the DNA with a DNA-binding resin improved the sensitivity. Admixtures of 0.1–0.5% can be detected in raw meat and 0.5–5% in autoclaved meat samples. The method can be adapted to detect any eukaryotic species for which species-specific DNA sequences are available. This method has proven its value in the routine inspection of meat samples by revealing more cases of deliberate or accidental species substitution and admixture than conventional techniques.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/sj.jim.2900541
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  • 4
    Electronic Resource
    Electronic Resource
    Can the spread of agriculture in Europe be followed by tracing the spread of the weed Silene latifolia. A RAPD study (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 1085-1090 
    Details
    ISSN: 1432-2242
    Keywords: Silene latifolia ; RAPD ; Agriculture ; Genetic drift ; DNA polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract On the basis of gene frequency data of three flavone glycosylating genes, populations of the agricultural weed Silene latifolia (Caryophyllaceae) in Europe can be divided into two chemical races: an eastern and a western race. Morphological data also show a clear east-west division. When the two datasets are combined at least nine different geographical races can be distinguished using cluster analysis. Because these observations are hard to explain by selection, it has been proposed that these different races probably originated as a consequence of migration during the spread of agriculture over Europe in the past. To discriminate between selection and genetic drift many more selectively neutral easy-to-score characters are needed. In order to test whether random amplified polymorphic DNAs (RAPDs) might be suitable for this purpose, we performed a small-scale RAPD analysis on 16 geographical different populations. Using Jaccard's coefficient of similarity, we calculated genetic distances by pair-wise comparisons of both unique and shared amplification products, and a dendrogram was subsequently constructed using an unweighted pair-group method with arithmetical averages (UPGMA). On the basis of the dendrogram two clusters were discerned that clearly coincide with the aforementioned east-west division in populations. As there has been little or no artificial selection on this weed, its migration routes may be a good reflection of the different geographical routes agriculture has taken. We propose that a phylogenetic analysis of RAPD data of many more populations may provide additional information on the spread of agriculture over Europe.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224053
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  • 5
    Electronic Resource
    Electronic Resource
    Can the spread of agriculture in Europe be followed by tracing the spread of the weed Silene latifolia. A RAPD study (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 1085-1090 
    Details
    ISSN: 1432-2242
    Keywords: Key words Silene latifolia ; RAPD ; Agriculture ; Genetic drift ; DNA polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  On the basis of gene frequency data of three flavone glycosylating genes, populations of the agricultural weed Silene latifolia (Caryophyllaceae) in Europe can be divided into two chemical races: an eastern and a western race. Morphological data also show a clear east-west division. When the two datasets are combined at least nine different geographical races can be distinguished using cluster analysis. Because these observations are hard to explain by selection, it has been proposed that these different races probably originated as a consequence of migration during the spread of agriculture over Europe in the past. To discriminate between selection and genetic drift many more selectively neutral easy-to-score characters are needed. In order to test whether random amplified polymorphic DNAs (RAPDs) might be suitable for this purpose, we performed a small-scale RAPD analysis on 16 geographical different populations. Using Jaccard’s coefficient of similarity, we calculated genetic distances by pair-wise comparisons of both unique and shared amplification products, and a dendrogram was subsequently constructed using an unweighted pair-group method with arithmetical averages (UPGMA). On the basis of the dendrogram two clusters were discerned that clearly coincide with the aforementioned east-west division in populations. As there has been little or no artificial selection on this weed, its migration routes may be a good reflection of the different geographical routes agriculture has taken. We propose that a phylogenetic analysis of RAPD data of many more populations may provide additional information on the spread of agriculture over Europe.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050234
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