ISSN:
1573-8221
Keywords:
brain Na,K-ATPase
;
inhibition by free radicals
;
ischemia
;
hydrogen peroxide
;
hypochlorite
;
S-nitrosoglutathione
;
Fe-dinitrozyl-cysteine complex
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract The susceptibility of Na,K-ATPase from bovine brain to various compounds containing active oxygen radicals is assessed. Sodium nitroprusside slightly inhibits Na,K-ATPase, while light-induced NOo radicals (controlled by the rate of ascorbate oxidation) have no effect on the enzyme. When added in concentrations equally effective in the ascorbate oxidation assay, hydrogen peroxide and sodium hypochlorite inhibit Na,K-ATPase by 70 and 25–30%, respectively. The Fe-dinitrosyl-cysteine complex is the most potent (K0.5=20 μM) inhibitor of Na,K-ATPase. It is demonstrated that different free oxygen radicals accumulated in the ischemic brain cause different kinds of damage to Na,K-ATPase.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02446761
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