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  • 1
    Monograph available for loan
    Monograph available for loan
    Hoboken, NJ : Wiley-Blackwell
    Associated volumes
    Call number: 20-2/M 12.0167
    In: Handbook of molecular microbial ecology
    Description / Table of Contents: Handbook of Molecular Microbial Ecology I: Metagenomics and Complementary Approaches is the first comprehensive reference covering the various metagenomics in a large variety of habitats, which could not previously have been analysed without metagenomic methodology. This Volume includes topics such as species designations in microbiology, metagenomics, consortia and databases, bioinformatics, microarrays, and other metagenomics applications. This reference is ideal for researchers in metagenomics, microbiology, environmental microbiology, those working on the Human Microbiome Project, microbial geneticists, molecular microbiology, and bioinformatics
    Description / Table of Contents: "Metagenomics has revolutionized microbiology and many associated health and environmental fields. This is the first comprehensive treatise covering the various omics in a large variety of habitats, which could not previously have been analysed without metagenomic methodology. This is a reference work for people in the field and those who would like to enter it"--
    Type of Medium: Monograph available for loan
    Pages: xxii, 761 S. , Ill., graph. Darst.
    ISBN: 9780470644799
    Series Statement: Handbook of molecular microbial ecology 1
    Note: Online-Ausg. ---〉 Bruijn, Frans J. de de: Handbook of molecular microbial ecology I
    Location: Reading room
    Branch Library: GFZ Library
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  • 2
    Unknown
    Dordrecht [u.a.] : Kluwer Acad. Publ.
    Call number: Bio-06-0035
    ISBN: 1402021763
    Branch Library: AWI Library
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 177 (1999), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A backpropagation neural network (BPN) was used to identify bacterial plant pathogens based on their genomic fingerprints. Genomic fingerprint data, comprised of complex DNA band patterns generated using BOX, enterobacterial repetitive intergenic consensus (ERIC) and repetitive extragenic palindromic (REP)-primers (rep-PCR), were used to train three independent BPNs. 10 Strains of the genus Xanthomonas, each with a characteristic host plant range, were identified correctly using the three trained BPNs. When tested with fingerprints of bacterial strains not present in the training sets, the rejection rate was 100%, using the three BPN classifiers combined. Thus, BPN protocols can be employed to generate a powerful computer-based system for the identification of pathogenic bacteria in the genus Xanthomonas.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: An 8551-bp plasmid, pFQ11, from Frankia alni strain CpI1 was sequenced. Its sequence was found to be very similar to that presented for pFQ31 from strain ArI3. Six potential protein-encoding open reading frames (ORFs) were identified, and transcriptional activity was shown within four of those regions of the plasmid by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. An earlier study reported that ORF EF of pFQ31, which is nearly identical to the 3′ 45% of ORF1 of pFQ11, is significantly similar to RepF. We found no such similarity. ORF2 and ORF3 predict products that are similar to a repressor protein and a partition protein, respectively. We found inverted repeats within and covering the start codon of ORF3; palindromic sequences and direct repeats between ORF3 and ORF4; and 3′ from ORF3, an AT-rich sequence that extensively overlaps the promoter region of a uvrB homolog in strain ArI3.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 22 (1997), S. 0 
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The gene encoding the green fluorescent protein (GFP) from the jellyfish Aequorea victoria was assembled into an expression cassette for bacteria by fusing it to the T7 gene10 ribosome binding site and the strong, constitutive promoter PpsbA from Amaranthus hybridus. By using Tn5-based transposon delivery systems, Pseudomonas fluorescens bacteria were chromosomally tagged with gfp. We demonstrate that expression of a single copy gfp gene is sufficient to permit the visualization of bacteria by epifluorescence and laser confocal microscopy and detection by flow cytometry. The green fluorescent phenotype was detectable in all growth phases even under nutrient-limited conditions.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1572-9699
    Keywords: Azorhizobium caulinodans ; coproporphyrin ; micro-aerobiosis ; excretion ; fixLJ ; fixK
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Azorhizobium caulinodans ORS571 was found to excrete moderate amounts of a fluorescent pigment into the culture medium in response to dissolved oxygen tensions below 1.0 kPa. The pigment was identified as coproporphyrin, on the basis of its optical and fluorescence spectra. FixLJ and fixK mutant derivatives of ORS571 were found to excrete 25-fold higher amounts of coproporphyrin under micro-aerobic conditions than the wild type strain. These observations suggest that A. caulinodans switches from an aerobic to an anaerobic coproporphyrinogen oxidase when the dissolved oxygen tension falls below 1.0 kPa and that the fixLJ and fixK genes are involved in the regulation of expression of the anaerobic coproporphyrinogen oxidase.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1573-5028
    Keywords: leghemoglobin ; gene expression ; site-directed mutagenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The involvement of the Sesbania rostrata glb3 gene promoter NICE (nodule-infected cell expression) element in root-enhanced expression of 5′-Srglb3-uidA-3′nos chimeric gene was investigated in transgenic Nicotiana tabacum plants. The full-length wild-type Srglb3 promoter directed root meristem-enhanced expression in transgenic tobacco plants. The expression pattern of nine selected Srglb3 promoter mutations in the NICE element was examined in transgenic tobacco plants and compared with the pattern observed in nodules of transgenic Lotus corniculatus plants. The results suggest that the highly conserved motifs in the NICE element play an important role in expression in roots of non-legume plants.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1617-4623
    Keywords: Leghemoglobin (lb) genes and sequences ; Sesbania rostrata ; Stem and root nodules ; Cis-elements ; Trans-acting factors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The primary structure of a leghemoglobin (lb) gene from the stem-nodulated, tropical legume Sesbania ostrata and two lb gene promoter regions was analysed. The S. rostrata lb gene structure and Lb amino acid composition were found to be highly conserved with previously described lb genes and Lb proteins. Distinct DNA elements were identified in the S. rostrata lb promoter regions, which share a high degree of homology with cis-active regulatory elements found in the soybean (Glycine max) lbc3 promoter. One conserved DNA element was found to interact specifically with an apparently universal, trans-acting factor present in nuclear extracts of nodules. These results suggest a conserved mechanism for nodule specific induction of lb genes in leguminous plants.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Molecular breeding 1 (1995), S. 419-423 
    ISSN: 1572-9788
    Keywords: binary vectors ; gus ; T-DNA ; Agrobacterium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We describe here a set of binary vectors suitable forAgrobacterium-mediated gene transfer and specially designed for studying plant promoters. These vectors are based on the use of thegus reporter gene, contain multiple unique restriction sites upstream of thegus gene, and minimal promoters for testing the effect of enhancers or activator elements. In addition, an intron-containinggus (uidA) gene was introduced into one of these vectors in order to examine reporter gene activity in tissues whereAgrobacterium contamination may be a problem or in transient expression assays.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1617-4623
    Keywords: Rhizosphere ; Nutritional mediator ; Periplasmic binding protein ; GntR-like regulator ; Dehydrogenase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Rhizopine (l-3-O-methyl-scyllo-inosamine, 3-O-MSI) is a symbiosis-specific compound, which is synthesized in nitrogen-fixing nodules of Medicago sativa induced by Rhizobium meliloti strain L5–30. 3-O-MSI is thought to function as an unusual growth substrate for R. meliloti L5–30, which carries a locus (mos) responsible for its synthesis closely linked to a locus (moc) responsible for its degradation. Here, the essential moc genes were delimited by Tn5 mutagenesis and shown to be organized into two regions, separated by 3 kb of DNA. The DNA sequence of a 9-kb fragment spanning the two moc regions was determined, and four genes were identified that play an essential role in rhizopine catabolism (mocABC and mocR). The analysis of the DNA sequence and the amino acid sequence of the deduced protein products revealed that MocA resembles NADH-dependent dehydrogenases. MocB exhibits characteristic features of periplasmic-binding proteins that are components of high-affinity transport systems. MocC does not share significant homology with any protein in the database. MocR shows homology with the GntR class of bacterial regulator proteins. These results suggest that the mocABC genes are involved in the uptake and subsequent degradation of rhizopine, whereas mocR is likely to play a regulatory role.
    Type of Medium: Electronic Resource
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