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Analysis of the transfer region of the Streptomyces plasmid SCP2* (1993)

Brolle, D.-F. ; Pape, H. ; Hopwood, D. A. ; [et al.]

Oxford, UK : Blackwell Publishing Ltd

Molecular microbiology 10 (1993), S. 0

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ISSN: 1365-2958

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology , Medicine

Notes: plJ903, a bifunctional derivative of the 31.4 kb low-copy number, conjugative Streptomyces plasmid SCP2, was mutagenized in Streptomyces lividans using Tn4560. Mutant plasmids differing in their transfer frequencies, chromosome mobilization abilities, pock formation, and complementation properties were isolated. The mutations defined five transfer-related genes, traA, traB, traC, traD and spd, clustered in a region of 9 kb. The deduced sequences of the putative TraA and TraB proteins showed no overall similarity to known protein sequences, but the phenotype of traA mutant plasmids and sequence motifs in the putative TraA protein suggested that it might be a DNA helicase.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1365-2958.1993.tb00912.x

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