ISSN:
1432-0983
Keywords:
Key words Rhizoctonia solani
;
ITS sequences
;
Asymmetric PCR
;
Phylogenetic tree
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The relatedness of nine isolates of Rhizoctonia solani, belonging to anastomosis group (AG) 4, and one isolate of AG1 was determined by comparative sequence analysis based on direct sequencing of PCR-amplified ribosomal DNA [the internal transcribed spacer (ITS) region and the 5.8 s ribosomal DNA]. The 5.8 s rDNA is completely conserved, but both ITS regions show variation among strains. AG1 was an outgroup based on anastomosis ability and RFLP analyses. Phylogenetic analyses based on the ITS sequences suggest that the analyzed AG4 strains can be divided into three groups that correlate with habitat and virulence.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002940050033
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