ISSN:
1573-5079
Keywords:
Ru-P2 carboxylase
;
long-term assay
;
short-term assay
;
specific activity
;
tobacco
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Ribulose 1,5-bisphosphate carboxylase (EC.4.1.1.39) has been obtained from Nicotiana tabacum leaf homogenates with specific activites from 0.5 to 0.8 µmol CO2 fixed (mg protein min)-1. These activities are reconciled with much lower, previously reported activities. The results suggest that if the tobacco enzyme is assayed under optimum conditions there is little difference in the intrinsic specific activities of tobacco and spinach ribulose 1,5-bisphosphate carboxylase. Several factors affecting activity measurements were examined.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00056260
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