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  • 1
    Electronic Resource
    Electronic Resource
    An ex vivo original test using radiotracers for evaluating haemocompatibility of tubular biomaterials
    Amsterdam : Elsevier
    Applied Radiation and Isotopes 45 (1994), S. 207-218 
    Details
    ISSN: 0969-8043
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0969-8043(94)90013-2
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  • 2
    Electronic Resource
    Electronic Resource
    In vitro assessment of endothelial cell adhesion mechanism on vascular patches (1999)
    Springer
    Journal of materials science 10 (1999), S. 807-810 
    Details
    ISSN: 1573-4838
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract Endothelial cell (EC) seeding of small caliber vascular grafts prior to their implantation has proved to significantly improve long-term patency in humans. We have previously demonstrated that a monolayer of EC could be obtained on type I collagen-coated knitted ultrathin polyster grafts (InterVascular, La Ciotat, France). Thus, the aim of the present work was to understand the nature of cell adhesion mechanisms involved in the cell /biomaterial interface, using HemaCarotid® (InterVascular) patches made of type I collagen-coated knitted ultrathin polyster (type I collagen is used to coat patches to attain low permeability). By means of quantitative attachment tests, adhesion blocking assays, RT–PCR for the expression of β1 integrin mRNA, indirect immunofluorescence with antivinculin antibody, we were able to show that EC are able to adhere to such surfaces by the means (non-unique) of cell surface receptors of the β1 integrin group. However, the latter are probably downregulated at the cell/biomaterial interface.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008971505127
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  • 3
    Electronic Resource
    Electronic Resource
    Preliminary report on a new composite material made of calcium phosphate, elastin peptides and collagens (1993)
    Springer
    Journal of materials science 4 (1993), S. 372-380 
    Details
    ISSN: 1573-4838
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: With a view to elaborating a bioactive bone substitute, the association of an artificial extracellular matrix, basically constituted of elastin-solubilized peptides (ESP) and type I+III collagens, with different types of calcium phosphates, was investigated. This paper describes the selective adsorption of ESP on some calcium phosphate samples, and the further association of the adsorbed peptides with type I+III collagens. A preliminary study of the material cytotoxicity was carried out, investigating the behaviour of human osteoblast cells in contact with the yielded composite material.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00122195
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  • 4
    Electronic Resource
    Electronic Resource
    Physico-chemistry and cytotoxicity of ceramics (1994)
    Springer
    Journal of materials science 5 (1994), S. 18-24 
    Details
    ISSN: 1573-4838
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: General cytotoxicity was assayed for ceramic (Al2O3, ZrO2/Y2O3, AIN, B4C, BN, SiC, Si3N4, TiB4, TiC, TiN) diamond and graphite powders, using 3T3 Balb/c permanent cell lines. Neutral red test was carried out in order to establish cell viability. Further investigations were undertaken on human differentiated cells (human umbilical venous endothelial cells): cell behaviour (MTT assay, total cell protein content) and differentiation (immunofluorescence) were studied. In both cases, no cytotoxic effect has been noticed. All the impurities contained at low concentration in these powders do not seem to present any effect. The correlation which has been previously observed between cytotoxicity-cell culture response and blood haemolysis for polymers has not been established here for ceramic powders. We conclude that all the ceramic powders tested here and therefore the corresponding bulk ceramics or ceramic coatings do not induce any cytotoxic effect.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00121148
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  • 5
    Electronic Resource
    Electronic Resource
    Ex vivo expansion of haematopoietic progenitors on an endothelialized hydroxyapatite matrix (1997)
    Springer
    Journal of materials science 8 (1997), S. 819-822 
    Details
    ISSN: 1573-4838
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract Autologous haematopoietic progenitor cell (HPC) transplantation is increasingly used to restore haematopoiesis after high-dose chemotherapy treatments. The present study was designed to analyse the ability of hydroxyapatite (HAP) seeded with endothelial cells (EC) to support the proliferation and differentiation of CD34+ HPC in static culture conditions. HAP is endothelializable as assessed by scanning electron microscopy and time-course DNA synthesis analysis using tritiated thymidine incorporated in EC isolated from human umbilical vein cord. Short-term coculture experiments in which CD34+ cells isolated from human cord blood were seeded on endothelialized HAP, were performed. Results show that endothelialized HAP is permissive to CD34+ cell expansion with a maximum expansion obtained between days 7 and 14 of coculture in the presence of IL-1 and IL-3 when compared with other experiments omitting either EC or interleukins. From morphological analyses, the expanded cell population mainly belonged to the myelocytic lineage with 33% mature cells (polymorphonuclear neutrophils and monocytes) at day 14 of coculture. The immature HPC could remain trapped within HAP while giving rise to a more mature progeny that exit from HAP microenvironment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018585201309
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  • 6
    Electronic Resource
    Electronic Resource
    Up-regulation of thrombomodulin is inducible on an endothelialized polyester (1997)
    Springer
    Journal of materials science 8 (1997), S. 877-879 
    Details
    ISSN: 1573-4838
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract The concept of endothelial cell seeding of vascular prostheses is designed as a method for improving long-term patency substitutes because endothelium is considered as the haemocompatible surface of reference. The assessment of the functionality of cells lining a biomaterial is thus of crucial importance. We have reported encouraging results concerning the ability of a polyester coated with albumin and chitosan (M 11) to be lined by a confluent monolayer of cultured human endothelial cells (EC). The aim of the present work was to study the expression of thrombomodulin (membrane glycoprotein responsible for anticoagulant activity) in EC lying on M.11 by anticoagulant activity and mRNA level with and without stimulation. Results obtained in basal conditions showed that EC on M.11 have a comparable expression of TM mRNA when compared to control (EC on tissue culture plates) despite a lower TM surface activity for EC on the biomaterial. In terms of ratio (stimulated cells to unstimulated ones) the response in activity for EC on M.11 is comparable to that of the control. These results indicate that cells lying on M.11 are able to respond to physiological-like stimuli, despite a tendency for these cells to express a procoagulant phenotype when compared with control EC.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018505905852
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  • 7
    Electronic Resource
    Electronic Resource
    Human tracheal epithelial cells in culture: a suitable model for testing the cytocompatibility of materials for endotracheal use (1993)
    Springer
    Journal of materials science 4 (1993), S. 327-336 
    Details
    ISSN: 1573-4838
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: It is well known that cuffs of endotracheal tubes can induce ischemic injuries on tracheal epithelium, as a result of mechanical hyperpressure caused by the cuff on the airway tissue. Whether or not material components are leached out and may provoke a direct toxic effect on the respiratory epithelium is much less clear. To study the cytocompatibility of such materials, we have developed an in vitro cell system using human tracheal epithelial cells, arising from trachea superficial biopsies. In culture, cells have been characterized by morphological and immunocytochemical criteria. Ultrastructural observations suggest that our culture conditions are permissive for the expression of both squamous and secretory phenotypes. We have assessed the cytocompatibility of a cuff towards epithelial cells, first, by an indirect test, and second by a direct test. By the indirect test, using material extracts, we did not find any toxic effect towards human airway epithelial cells of the cuff components. By a direct test, we found a slight cell lysis after a 24 h incubation. Our study shows that this human tracheal epithelial cell system is a useful and relevant model which could be used in a quality control procedure for testing the cytocompatibility of materials for endotracheal use.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00122289
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  • 8
    Electronic Resource
    Electronic Resource
    Endothelial cell compatibility testing of various prosthetic surfaces (1994)
    Springer
    Journal of materials science 5 (1994), S. 808-812 
    Details
    ISSN: 1573-4838
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract Numerous methods are proposed to reduce the surface thrombogenicity of vascular prostheses, among them endothelialization of the lumen which has had clinical application since 1985. One of the problems is to collect enough cells to rapidly obtain a complete monolayer at the time of implantation. Thus, material improvements are necessary to enhance cell adhesion and spreading. A collaboration with the Bakoulev Institute (Moscow) gave us the opportunity to study the cytocompatibility of carbon coated materials (PAN and Vitlan®), polyester coated with albumin and/or synthetic polysaccharide. Studies were carried out with cultured human umbilical vein endothelial cells (HUVEC). Two steps are distinguished: indirect tests (medium added with materials extracts), then direct tests (cells directly seeded onto materials). Neither PAN, Vitlan® nor polyesters extracts have provoked a toxic effect on HUVEC. Concerning attachment on materials, a maximum of 60% of seeded cells is reached. Cells could proliferate and confluency is obtained between days 5 and 10 for the best materials. SEM corroborated these results. On polysaccharide-coated polyester (M. 11), HUVEC produced significant levels of vWF after thrombin stimulation (ELISA assay): vWF was functional (ristocetin cofactor activity). In conclusion, PAN and M. 11 gave encouraging results and further studies remain to be investigated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00213139
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  • 9
    Electronic Resource
    Electronic Resource
    Optimization of use of UEA-1 magnetic beads for endothelial cell isolation (1996)
    Springer
    Cell biology and toxicology 12 (1996), S. 189-197 
    Details
    ISSN: 1573-6822
    Keywords: endothelial cell isolation ; magnetic beads ; Ulex europaeus agglutin-1-coated Dynabeads
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Most endothelial cells (EC) in the body belong to the microvasculature. Isolation and subsequent culture of these microvessel EC contributes greatly to our understanding of the heterogeneity and vascular specificity that exist between one organ site and another. However, a major obstacle is the overgrowth of contaminating cells (fibroblasts, pericytes, smooth-muscle cells) in cultures. Since 1990 the use of magnetic beads in combination with either a lectin, Ulex europaeus agglutinin-1 (UEA-1), or a monoclonal antibody has represented a powerful tool for the isolation/purification of microvessel EC. In the former case, operative conditions remain to be optimized to obtain pure cultures of EC. We have performed studies to optimize conditions of use for magnetic beads coated with UEA-1. Incubating beads with cells, the influences are studied of time, temperature, cell concentration, and number of beads per target cell for two cell types, human umbilical vein EC (HUVEC) and skin fibroblasts (HSF), either isolated or mixed. The effect of the last parameter was also checked on the behavior of cells undergoing proliferation after isolation. Results, expressed as isolation efficiency (from 40% to 90%) allowed us to select a 15-min incubation time at 4°C with rotary agitation, an optimal concentration of 4 x 105 cells/ml, and an optimal cell:bead ration of 1:3. From a mixed cell population and in these conditions, even very low HUVEC:HSF proportions of 2.5:97.5 allowed us to obtain a pure HUVEC population in subsequent culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00438144
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  • 10
    Electronic Resource
    Electronic Resource
    Ability of various inserts to promote endothelium cell culture for the establishment of coculture models (1996)
    Springer
    Cell biology and toxicology 12 (1996), S. 207-214 
    Details
    ISSN: 1573-6822
    Keywords: coculture ; differentiation ; endothelial cell ; human cell culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract To select an insert suitable for human umbilical vein endothelial cell (HUVEC) culture, we compared several available inserts of 0.2 to 0.45 μm porosity: Cellagen (ICN), Transwell-COL (Costar), Millicell-HA and CM (Millipore), Anopore (Nunc), Cyclopore (Falcon) in comparison with a control surface (Thermanox). The requirements were: (i) to promote attachment, adhesion and proliferation of HUVEC (judged by [3H]thymidine incorporation into DNA at days 1, 3, 7); (ii) to allow HUVEC visualization by inverted, fluorescence microscopy for uptake of DiI-Ac-LDL and scanning electron microscopy, performed at day 9 after seeding. Because Transwell and Cellagen are collagen precoated and CM has to be coated for cell culture, we performed collagen coating (types I + III or IV) for non-pretreated inserts for the purpose of comparison. Our preferences comprise Transwell-COL, Cyclopore not coated or coated (whatever the collagen type), and Cellagen. However, on a quality/price ratio criterion, Cyclopore, even uncoated, is the insert of choice. The HA, CM and Anopore inserts, even coated, do not allow HUVEC growth but do not alter positive uptake of acetylated LDL.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00438147
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