ISSN:
1435-0653
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Triticum aestivum L.) cultivars using 117 restriction fragment length polymorphism (RFLP) probes, 16 amplified fragment length polymorphism (AFLP) primer combinations, and 21 simple sequence repeat (SSR) primer pairs, (ii) investigated the correlation between coancestry (f ) and genetic similarity (GS) estimated from molecular markers, and (iii) evaluated the use of f and GS for predicting the genetic variance (σ2 g) within sets of 22 F4∝n (n = 7 or 8) lines derived from 30 crosses between these winter wheat cultivars. The average polymorphic information content (PIC) for polymorphic bands was not significantly different between the three marker systems (0.30 ≤ PIC ≤ 0.33), whereas the marker index was low for RFLPs and SSRs but high for AFLPs. Estimates of f between all 55 cultivar combinations ranged from 0.01 to 0.52. GS-values varied between 0.52 and 0.89 for RFLPs, between 0.40 and 0.83 for AFLPs, and between 0.16 and 0.91 for SSRs. The Mantel Z test statistic revealed no common pattern between the four dendrograms obtained by cluster analyses. Significant (P 〈 0.05) correlations among f, GS-RFLP, GS-AFLP, and GS-SSR were detected only for related parent combinations (f ≥ 0.10). For all seven traits analyzed, estimates of σ2 g were not significantly associated with any measure of GS between parents. On the basis of these results, we recommend AFLPs for fingerprinting wheat cultivars. However, predicting the progeny variance σ4 g remains an unsolved problem.
Type of Medium:
Electronic Resource
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