ISSN:
0006-3592
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Evidence for generation of the plateau in oxygen uptake during exertion of carbonaceous BOD by pure cultures was sought by making long term Warburg studies using glucose and phthalic acid as substrates. One organism, tentatively identified as Escherichia intermedia, of the 57 tested, exhibited a plateau. Detailed studies on this organism indicated that generation of the plateau depended upon the type of substrate and to some extent upon the initial cell concentration employed, but not upon substrate concentration. Oxygen utilization during the second stage was accompanied by metabolism of secondary extracellular carbon source(s) produced by the cells during metabolism of the original extracellular carbon source (glucose) in the first phase of O2 uptake. The secondary substrate was registered as volatile acids. Selective inhibition of protein synthesis at various times during oxygen uptake led to the conclusion that the plateau occurred because of the need for an induction period before the secondary substrate could be metabolized. Using this organism, it was also found that a plateau could be generated during endogenous respiration. This secondary O2 utilization was accompained by uptake of organic materials excreted into the medium by the cells during metabolism of endogenous carbon sources.
Additional Material:
7 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bit.260070307
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