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  • 1
    Electronic Resource
    Electronic Resource
    In Vitro Response to Fusarium Elicitor and Toxic Substances in Crosses between Resistant and Susceptible Carnation Cultivars (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 98 (1987), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: To assess the correlation between in vivo resistance to F. oxysporum f. sp. dianthi and in vitro behaviour, calli from several resistant and susceptible Mediterranean carnation cultivars and F1 progenies of crosses were tested both for the ability to grow on a medium containing crude culture filtrate of F. oxysporum and to produce phytoalexins following treatment with cell wall components (elicitor) of the same fungus. The results show no significant correlation between in vivo resistance and in vitro tolerance to culture filtrate of the fungus, while there was a good correlation in die case of phytoalexin production. Moreover, the character phytoalexin production behaved as a dominant in the crosses between a resistant and a susceptible cultivar.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1987.tb01141.x
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  • 2
    Electronic Resource
    Electronic Resource
    Impact of positioning and number of polarimetric measurements on the plasma reconstruction in fusion devices (2001)
    Bradford : Emerald
    Compel 20 (2001), S. 216-232 
    Details
    ISSN: 0332-1649
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: The identification of plasma parameters from different sets of measurements is a key topic in the thermonuclear fusion research. Most of the information relevant to the plasma shape and position control is usually gained via external magnetic measurements, but information related to internal distribution of current density is not accessible in this way. Other possible measurements are available. In this paper a performance analysis is done with respect to the adoption of polarimetric measurements.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1108/03321640110359949
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  • 3
    Electronic Resource
    Electronic Resource
    Combined reconstruction techniques for geometrical and magnetic characteristics of thermonuclear plasmas (2001)
    Bradford : Emerald
    Compel 20 (2001), S. 699-712 
    Details
    ISSN: 0332-1649
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: The equivalent currents method has proven to be particularly effective in the identification of plasma boundary in Tokamak fusion devices. Anyway, the ill-posedness of the mathematical model to be inverted calls for the adoption of suitable regularization techniques to be adopted, in particular to reduce the influence of the measurement errors. In this paper the equivalent currents method is illustrated, together with some details on its application to the plasma identification. In addition, two algorithms for the optimal choice of the representation basis are presented, together with a discussion about the obtained numerical results.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1108/03321640110393699
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  • 4
    Electronic Resource
    Electronic Resource
    CAMAC branch driver: A particular solution
    Amsterdam : Elsevier
    Nuclear Instruments and Methods 112 (1973), S. 527-532 
    Details
    ISSN: 0029-554X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0029-554X(73)90173-0
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  • 5
    Electronic Resource
    Electronic Resource
    Light and developmental regulation of the Anp-controlled anthocyanin phenotype of bean pods (1993)
    Springer
    Theoretical and applied genetics 87 (1993), S. 1-8 
    Details
    ISSN: 1432-2242
    Keywords: Phaseolus vulgaris ; Anthocyanin biosynthesis ; Genetic, light and developmental control ; PAL and CHS mRNA steady state level
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In the presence of the dominant allele of the Anp gene, bean pods present a purple-mottled phenotype. The purple pigmentation is variable from cell to cell in the pod epidermal layer and develops as a random mosaic. Three anthocyanidins, delphinidin, petunidin and malvidin, are involved in this purple pigmentation. Anthocyanins accumulated in vacuoles; anthocyanoplasts and cristal bodies were also observed occasionally. A developmental switch is a prerequisite for anthocyanin accumulation in the pods. This does not occur before day 4 after pollination and is controlled by light in competent pods. mRNAs for PAL, CHS, CHI, DFR and UFGT are induced in the pods, indicating that the general anthocyanin biosynthetic pathway is well conserved at both the biochemical and molecular levels in this species. mRNA steady-state level studies of PAL and CHS suggest that the light regulation occurs at the transcriptional level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223735
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  • 6
    Electronic Resource
    Electronic Resource
    Modification of competence for in vitro response to Fusarium oxysporum in tomato cells. II. Effect of the integration of Agrobacterium tumefaciens genes for auxin and cytokinin synthesis (1994)
    Springer
    Theoretical and applied genetics 88 (1994), S. 89-96 
    Details
    ISSN: 1432-2242
    Keywords: Lycopersicon esculentum ; Fusarium oxysporum f. sp. ; lycopersici ; Agrobacterium hormone synthetic genes ; Defense response
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have studied the effect of a change in the endogenous hormone equilibria on the competence of tomato (Lycopersicon esculentum) cells to defend themselves against the fungal pathogen Fusarium oxysporum f. sp. lycopersici. Calluses from cvs ‘Davis’ and ‘Red River’, respectively resistant and susceptible to Fusarium and transgenic for an auxin- or cytokinin-synthesizing gene from Agrobacterium tumefaciens, were used. The integration of Agrobacterium hormone-related genes into susceptible cv ‘Red River’ can bring the activation of defense processes to a stable competence as assessed by the inhibition of mycelial growth in dual culture and gem-tube elongation of Fusarium conidia, the determination of callose contents, peroxidase induction and ion leakage in the presence of fusaric acid. This is particularly true when the transformation results in a change of phytohormone equilibria towards an higher cytokin in concentration. On the contrary, in resistant cv ‘Davis’ the inhibition of both fungal growth in dual culture and conidia germination is higher when the hormone balance is modified in favour of the auxins. No significant effect was observed for ion leakage and peroxidase induction, probably because of a constitutive overproduction of cytokinins in ‘Davis’ cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222399
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  • 7
    Electronic Resource
    Electronic Resource
    Correlations between in vivo resistance to Fusarium and in vitro response to fungal elicitors and toxic substances in carnation (1985)
    Springer
    Theoretical and applied genetics 70 (1985), S. 42-47 
    Details
    ISSN: 1432-2242
    Keywords: Dianthus caryophyllus ; Fusarium oxysporum ; Physiological resistance genetics ; Tissue cultures ; Phytoalexin metabolism ; Toxins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary With the aim of ascertaining the existance of a correlation between in vivo resistance to Fusarium oxysporum f. sp. dianthi and in vitro response to fungal elicitors and toxic substances, phenylalanine ammonialyase and phytoalexin accumulation, on one hand, and resistance to culture filtrate, on the other, were assayed in “in vitro” cultures of three susceptible and four resistant Dianthus caryophyllus cultivars. Cultivars showing varying degrees of resistance in vivo either tolerated higher culture filtrate concentrations (‘Niki’) or showed high PAL activity and phytoalexin production when treated with Fusarium elicitor (‘Duca’), or responded positively to both treatments (‘Mei-Ling’, ‘Pulcino’). No such responses were shown in tissue cultures of susceptible cultivars. The differential response to the fungal elicitor seemed to be highly specific as genetic differences between cultivars were not observed in tissue cultures treated with other biotic (Phytophthora infestans) and abiotic (HgCl2) elicitors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00264480
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  • 8
    Electronic Resource
    Electronic Resource
    Isolation of tomato cell lines with altered response to Fusarium cell wall components (1987)
    Springer
    Theoretical and applied genetics 75 (1987), S. 37-40 
    Details
    ISSN: 1432-2242
    Keywords: Elicitor ; Fusarium oxysporum ; in vitro selection ; Pathogen resistance ; Phytoalexin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary To obtain Tomato cell lines with an altered capacity to respond to heat-released cell wall components (elicitor) of a tomato pathogen (Fusarium oxysporum f. sp. lycopersici), positive and negative selection experiments, using BUdR enrichment techniques, were carried out on suspension cultures of the susceptible, low phytoalexin producer cultivar Red River. Both high and low phytoalexin producing clones were isolated. Further tests demonstrated that not all phytoalexin-producing clones were more susceptible to the elicitor toxic effect, and that they were altered also in the speed of response to fungal cell wall components. Cells selected with Fusarium elicitor showed the same behaviour when challenged by Phytophthora infestans elicitor, thus suggesting in this case lack of specificity. The results are finally discussed with a view to using the technique both as a tool to study the genetics and physiology of hostparasite interactions and as a possible new method for the selection of pathogen resistant genotypes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00249139
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  • 9
    Electronic Resource
    Electronic Resource
    A mitochondrial transcript with homology to the 3′ end of the chloroplast psbA gene is present only in the atrazine-resistant biotype of Chenopodium album (1988)
    Springer
    Theoretical and applied genetics 75 (1988), S. 291-297 
    Details
    ISSN: 1432-2242
    Keywords: ct psbA gene ; mtDNA ; Gene expression ; Atrazine resistance ; Promiscuous DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The 3′ end of the chloroplast (ct) psbA gene is present in the mitochondrial genome of Chenopodium album and is expressed in mitochondria from atrazine-resistant plants. Short specific chloroplast psbA gene probes reveal a 270 bp homolog with the 3′ end of the chloroplast psbA gene. The homology starts close to the codon 264 of this gene; this codon is known to be involved in atrazine resistance when mutated in the chloroplast DNA. Comparison of susceptible and resistant plants has not given evidence of modification in the mitochondrial (mt) genome; however, the homologous 3′ end of the ct psbA gene present in the mitochondria is expressed as part of a 0.8 kb transcript only in atrazine-resistant plants and not in susceptible ones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00303967
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  • 10
    Electronic Resource
    Electronic Resource
    Modification of competence for in vitro response to Fusarium oxysporum in tomato cells. I. Selection from a susceptible cultivar for high and low polysaccharide content (1994)
    Springer
    Theoretical and applied genetics 87 (1994), S. 988-995 
    Details
    ISSN: 1432-2242
    Keywords: Lycopersicon esculentum ; Fusarium oxysporum f.sp. lycopersici ; Polysaccharides ; Active defence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Plant cell walls play a major role in the outcome of host-parasite interactions. Wall fragments released from the plant, and/or the fungal pathogen, can act respectively as endogenous and exogenous elicitors of the defence response, and other wall components, such as callose, lignin, or hydroxyproline-rich glycoproteins, can inhibit pathogen penetration and/or spreading. We have previously demonstrated that calli from tomato cultivars resistant in vivo to Fusarium oxysporum f.sp. lycopersici show a high amount of polysaccharides in vitro. The aim of the present work was to assess the possible role of polysaccharide content and/or synthetic capacity in determining the competence of plant cells for active defence. For this purpose, tomato cell clones with increased and decreased polysaccharide (FL+, FL-) and callose (A+, A-) content have been selected by means of specific stains as visual markers and tested for the effect of these changes on the extent of response to Fusarium. The analysis of several parameters known to be indicative of active defence (cell browning after elicitor treatment, peroxidase and β-glucanase induction and inhibition of fungal growth in dual culture) clearly shows that FL+ and A+ clones have acquired an increased competence for the activation of defence response. The results are thoroughly discussed in terms of an evaluation of the relative importance of constitutive and/or inducible polysaccharide synthetic capacity for plant response to pathogens, and their possible regulation by plant physiological backgrounds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225793
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