ISSN:
1573-4943
Keywords:
Δ5-3-ketosteroid isomerase
;
Δ6-testosterone succinyl agarose
;
photoaffinity labeling
;
solid-phase photoactivatable reagent
;
ultraviolet irradiation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract The nature of the products formed during the photoinactivation of Δ5-3-ketosteroid isomerase in the presence of the solid-phase photoaffinity reagent Δ6-testosterone succinyl agarose has been investigated after ultraviolet irradiation. The polypeptide products eluted from the agarose phase by sodium cholate, sodium dodecyl sulfate, and pH 10.5 triethylamine buffer have been characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis, pH 4–6 gel isoelectric focusing, and amino acid analysis. The amino acid compositions of the cholate eluted and SDS eluted products are found to be similar to that of native isomerase, whereas the covalently bound polypeptide eluted by pH 10.5 triethylamine possesses a distinetly different composition. Digestion of the covalently bonded isomerase polypeptide with trypsin yields an agarose-bound peptide fraction that has been characterized by its amino acid composition. This composition is different from that of the undigested covalently bound polypeptide and suggests that the site of covalent attachment lies somewhere between residues 28 and 45 of the isomerase polypeptide.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01024839
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