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  • 1
    Electronic Resource
    Electronic Resource
    Confirmation of the role of auxin and calcium in the late phases of adventitious root formation (1998)
    Springer
    Plant growth regulation 26 (1998), S. 191-194 
    Details
    ISSN: 1573-5087
    Keywords: adventitious rooting ; anti-auxins ; lanthanum chloride ; poplar shoots
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Poplar shoots raised in vitro were induced to root by incubation on an auxin (NAA) containing medium for 7 h. After 13 days on an auxin-free medium, 97% of the treated shoots had rooted. The introduction of known antiauxins (PCIB, PBA, POAA) into the rooting expression auxin-free medium, after the 7-h induction by NAA, completely (PCIB and PBA) or severely (POAA) inhibited rooting. The exclusion of calcium from the expression auxin free medium reduced the percentage of rooting by about 42%. The inhibition was still higher in the presence of EGTA, a calcium chelator. Lanthanum chloride, a calcium channel blocker, also completely inhibited rooting, when incorporated into the auxin free medium, with or without calcium. These results support previous hypotheses about the implication of both endogenous auxin and calcium in the late phases of the adventitious rooting process.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006182801823
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  • 2
    Electronic Resource
    Electronic Resource
    The relationship between rooting of poplar shoots and ATPase activity of their crude microsomal vesicles (1998)
    Springer
    Plant growth regulation 24 (1998), S. 43-48 
    Details
    ISSN: 1573-5087
    Keywords: ATPase ; auxin ; dicyclohexylcarbodiime ; microsomes ; poplar shoots ; rooting ; vanadate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Poplar shoots raised in vitro were induced to root by a 7 h passage on an auxin (1-naphthaleneacetic acid) medium. The percentage of rooting was reduced from ± 97% to ± 47% when vanadate (200 µM) was included in the auxin medium. Introduction of vanadate in the medium without auxin after the 7 h induction on auxin medium, did not inhibit rooting but affected only the development of the roots produced. The Mg2+-dependent ATPase activity of the microsomal vesicles of poplar shoots was increased after 7 h induction on rooting medium and corresponded to an increase in the Vmax of the enzyme. Results from experiments using some inhibitors of the polyamine metabolism suggested that this pathway was not involved in the increase of this activity. The auxin had no effect on the in vitro ATPase activity at any concentration tested except at about 2 mM where it was inhibitory, probably due to a change in the conformation of the enzyme. The transient increase of indole-3-acetic acid during rooting induction could be responsible for the increase in the level of the enzyme. The inhibition of root formation and growth by vanadate indicates strongly that the ATPase activity may be necessary for the induction and expression of rooting.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005998314650
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    Paper (German National Licenses)
    Fulltext
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