ISSN:
1662-8985
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
A new, direct method has been developed to measure the adhesion forces of bovineserum albumin (BSA) on surfaces by using Atomic Force Microscopy (AFM) in liquid environment.We were able to measure interactions between proteins and substrate surface in PBS solutiondirectly without any modification to the substrate or the AFM tip. Two different surfaces have beenused in the experiments: mica (hydrophilic surface) and polystyrene (hydrophobic surface). Theresults show that a polystyrene surface is more adhesive to BSA than a mica surface. This isconsistent with previous research, which assessed that hydrophobic surfaces enhance proteinadhesion but hydrophilic surfaces do not, demonstrating the effectiveness of the technique
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/40/transtech_doi~10.4028%252Fwww.scientific.net%252FAMR.32.49.pdf
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