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  • 1
    Electronic Resource
    Electronic Resource
    Effect of coal rank on structure of tars from low-temperature pyrolysis of Canadian coals (1984)
    s.l. : American Chemical Society
    Industrial and engineering chemistry 23 (1984), S. 134-140 
    Details
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/i300013a027
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  • 2
    Electronic Resource
    Electronic Resource
    Reduction of 1,3-dimethyl-5-(p-nitrophenylimino)barbituric acid by thiols. A high-velocity flavin model reaction with an isolable intermediate (1979)
    s.l. : American Chemical Society
    Journal of the American Chemical Society 101 (1979), S. 1890-1893 
    Details
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ja00501a049
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  • 3
    Electronic Resource
    Electronic Resource
    Hyaline mutants from Verticillium dahliae, an example of selection and characterization of strains for host–parasite interaction studies (1998)
    Oxford, U.K. and Cambridge, USA : Blackwell Science Ltd
    Plant pathology 47 (1998), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The objective of the present work was to select stable well-characterized strains of Verticillium dahliae that could be used as biological tools in genetic and plant–microbe interaction studies. Hyaline mutants, known for their stability in pathogenicity were chosen for the study. Diversity in pathogenicity was found among hyaline subclones obtained from a defoliating wild-type clone, but not within those from nondefoliating ones. Most subclones from the defoliating clone had parental pathotypes, but one (V7-2) exhibited weak pathogenicity. This subclone (V7-2), together with a highly virulent one (V7-7) deriving from the same defoliating parent clone (7), were selected for further characterization, because of their differences in pathogenicity. When studied on the basis of their growth requirements, the two subclones expressed marked differences. V7-7 grew better than V7-2 over a wider range of temperature conditions. Both subclones grew similarly in media supplemented with NH4 as nitrogen source, but in those with NO3, V7-7 grew more vigorously than V7-2 and only the former could grow when NO2 was used. In spite of these differences, the two subclones were found to belong to the same vegetative compatibility group, confirming their genetic proximity. These results highlight the physiological and genetic complexity inherent in V. dahliae. In view of their characteristics, the clones obtained in this study should prove to be valuable tools in furthering the understanding of genetic and host–V. dahliae interactions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3059.1998.00257.x
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  • 4
    Electronic Resource
    Electronic Resource
    Systemic induction of phytoalexins in cucumber in response to treatments with fluorescent pseudomonads (2000)
    Oxford, UK : Blackwell Science Ltd
    Plant pathology 49 (2000), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The Pseudomonas putida isolate BTP1 and its sid− mutant M3 were recently reported to protect cucumber against Pythium aphanidermatum root rot. This protection was mainly associated with an accumulation of antifungal phenolics in the treated roots. In this study, analyses of root extracts from split-root experiments showed that these phytoalexins were produced systemically. Indeed, several antifungal molecules accumulated similarly in both treated and nontreated root parts of plants protected against P. aphanidermatum with BTP1 or M3. In addition, analyses of leaf samples also revealed increased amounts of fungitoxic molecules in PGPR-treated plants, although the nature of these molecules appeared to be different from those detected in roots. The antifungal compounds isolated both from roots and leaves were mainly detected in acid-hydrolyzed extracts containing aglycones. These results suggest that PGPR can elicit phytoalexins systemically in cucumber and that the overall defence response is not based on a single phytoalexin but is chemically complex and organ-specific.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3059.2000.00468.x
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  • 5
    Electronic Resource
    Electronic Resource
    IMMUNOCHEMOTHERAPY OF LUNG CANCER (1976)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 277 (1976), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1976.tb41720.x
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  • 6
    Electronic Resource
    Electronic Resource
    Interaction of NO and NO2 on 12-tungstophosphoric acid (1995)
    Springer
    Catalysis letters 32 (1995), S. 371-378 
    Details
    ISSN: 1572-879X
    Keywords: nitrogen oxides ; 12-tungstophosphoric acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract On exposure of the solid 12-tungstophosphoric acid (H3PW12O40· nH2O (HPW)) to NO2 at 150°C NO2 is sorbed, while HNO3 is produced. NO2 is found to penetrate into the bulk structure whose reaction occurs with the water hydrogen-bonded to the protons to produce HNO3. The NO2 retained on the catalyst is stoichiometrically equivalent to the total number of protons on and in the solid. This, together with infrared spectra, suggests that NO2 is associating (1/1) with the protons, producing a nitronium salt of the solid acid. Although insignificant quantities of NO are taken up by HPW, after exposure to NO2, approximately stoichiometric quantities of NO are sorbed and the infrared spectra show the presence of N2O3.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00813231
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  • 7
    Electronic Resource
    Electronic Resource
    Purification and characterization of new fatty acids with antibiotic activity produced bySporothrix flocculosa (1996)
    Springer
    Journal of chemical ecology 22 (1996), S. 405-413 
    Details
    ISSN: 1573-1561
    Keywords: Sporothrix flocculosa ; antibiotics ; fatty acids ; biocontrol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Chloroform extracts from a culture broth of the biocontrol fungusSporothrix flocculosa were separated by thin-layer chromatography (TLC). Compounds with antifungal activity were revealed with theCladosporium cucumerinum bioassay on TLC plates and were isolated by using silica gel column, preparative TLC, and reverse-phase high-performance liquid chromatography (RP-HPLC). They were identified by standard methods of nuclear magnetic resonance (NMR), gas chromatography (GC), mass spectrometry (MS), and infrared spectrum (IR). In addition to an active molecule previously described, two new fatty acids with antibiotic activity were characterized, 9-heptadecenoic acid and 6-methyl-9-heptadecenoic acid, the latter displaying the strongest activity among all three active molecules. This high number of active metabolites produced byS. flocculosa would explain its rapid and strong activity as a biocontrol agent of powdery mildews. In this context, screening ofS. flocculosa isolates for increased production of these molecules should help in selection of candidates with superior biocontrol properties.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02033644
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  • 8
    Electronic Resource
    Electronic Resource
    Synthesis and Biological Characterization of (Z)-9-Heptadecenoic and (Z)-6-Methyl-9-Heptadecenoic Acids: Fatty Acids with Antibiotic Activity Produced by Pseudozyma flocculosa (2000)
    Springer
    Journal of chemical ecology 26 (2000), S. 987-1000 
    Details
    ISSN: 1573-1561
    Keywords: Fatty acids ; Pseudozyma flocculosa ; powdery mildew ; antibiosis ; biocontrol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Difficulties in isolating and purifying antibiotic fatty acids from culture filtrates of Pseudozyma flocculosa, a biocontrol agent against powdery mildew, have been limiting factors in studying the properties and understanding the mode of action of the biocontrol agent. We report a new protocol for synthesizing (Z)-9-heptadecenoic and for the first time synthesis of (Z)-6-methyl-9-heptadecenoic acids, two antibiotic fatty acids produced by P. flocculosa. This allowed reproducible and quantifiable means of assaying biological activity of the molecules. In these bioassays, both molecules exhibited antifungal activity corresponding to their expected potency. These new developments should facilitate further studies aimed at deciphering the ecological properties of P. flocculosa.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005464326573
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  • 9
    Electronic Resource
    Electronic Resource
    Effect of oxygen concentration on plant growth, lipidperoxidation, and receptivity of tomato roots to Pythium F under hydroponic conditions (1997)
    Springer
    European journal of plant pathology 103 (1997), S. 255-264 
    Details
    ISSN: 1573-8469
    Keywords: minor root pathogens ; oxidative stress ; peroxidation of membrane lipids ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effects of the nutrient solution oxygenation on the growth of tomato plants and colonization of plant roots by Pythium F707, an isolate with filamentous non-inflated sporangia, were investigated under hydroponic conditions. Lipoperoxidation was also estimated determining lipoxygenase activity and conjugated dienes. Tomato plants were grown under either a high (11-14%; Air treatment), a moderate (5.8-7%; Control) or a low (0.8-1.5%; Nitrogen treatment) oxygen concentration and inoculated or not with the pathogen. The high oxygen treatment resulted in a marked increase in plant growth, as measured by shoot and root weights. Root and top weights were about the same in the nitrogen-treated plants and the controls. In these treatments, plants started showing typical symptoms of root decay and infection within 6 days after inoculation with Pythium F, while highly oxygenated plants remained healthy throughout the experiment and showed a significant decrease in root colonization by the pathogen, as estimated by the immunoenzymatic staining procedure and isolation of thalles on selective medium. Nitrogen-treated plants and controls produced higher amounts of conjugated dienes and revealed increased lipoxygenase activities in comparison with highly oxygenated plants. These differences were more pronounced after inoculation with the pathogen. Our data suggest that increases in lipoxygenase activity detected in the present study in tomato roots grown under oxygen stress and inoculated with Pythium F may lead to degradation and disorganization of membrane lipids. That disorganization may facilitate root colonization by the pathogen and appearance of decay.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008691226213
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  • 10
    Electronic Resource
    Electronic Resource
    Comparative effects of temperature and humidity on the activity of three potential antagonists of rose powdery mildew (1991)
    Springer
    European journal of plant pathology 97 (1991), S. 203-208 
    Details
    ISSN: 1573-8469
    Keywords: Biological control ; hyperparasites ; Sphaerotheca pannosa var.rosae ; Stephanoascus spp. ; Tilletiopsis washingtonensis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Three reported antagonists of cucumber powdery mildew,Stephanoascus flocculosus, Stephanoascus rugulosus, andTilletiopsis washingtonensis, were tested and compared under different environmental conditions for their potential for controlling rose powdery mildew, caused bySphaerotheca pannosa var.rosae. Under controlled conditions in vitro, all three fungi induced a rapid collapse of conidia, conidiophores and hyphae ofS. pannosa var.rosae on detached leaflets of miniature roses within 48 h following their application, as observed under a SEM. Both temperature and relative humidity (r.h.) affected the activity of the antagonists differently. The colonization of powdery mildew was maximal at 26 °C, especially forSt. rugulosus andT. washingtonensis. Maximal colonization was achieved at the highest r.h. tested (90%) for all three antagonists but onlySt. flocculosus maintained a colonization of 80% or better under lower r.h. These observations stress the importance of considering environmental conditions when assessing the activity of antagonistic microorganisms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01989818
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