ISSN:
1420-9071
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary A technique was developed for perfusing free airway cells (FAC) obtained by guinea-pig bronchoalveolar lavage. 10×106 FAC (mostly macrophages) were placed on the filter of a Nuclepore chamber perfused with Tyrode's solution or with Eagle's Minimal Essential Medium (MEM); the effluent was collected at intervals and the release of prostaglandin E2 by the perifused cells was used as an index of the inflammatory reaction to toxicants. Zymosan, asbestos fibers and soluble toxic agents stimulated the synthesis of prostaglandins while indomethacin abolished it. Our technique of FAC perifusion allows the sequential study of biochemical events involved in macrophage defense mechanisms as well as providing a simple test for the evaluation of various toxicants.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01955404
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