ISSN:
1573-5001
Keywords:
Lysozyme
;
Coupling constants
;
χ1 angles
;
COSY cross peaks
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Summary A method for estimating αCH-βCH coupling constants from the shape and fine structure of NH-αCH fingerprint-region cross peaks of COSY spectra is presented. Spectral simulations have been used to analyse the effect of variations in 3JNH-αCH, 3JαCH-βCH, linewidths and digital resolution on the appearance of NH-αCH COSY cross peaks. On the basis of these simulations a set of rules for broadly categorising experimental NH-αCH cross peaks according to αCH-βCH coupling constants has been devised. The method has been applied to the analysis of NH-αCH cross peaks of hen lysozyme. The results are compared to previous measurements of αCH-βCH coupling constants using E.COSY techniques.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00176008
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