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  • 1
    Electronic Resource
    Electronic Resource
    A stress-induced, developmentally regulated, highly polymorphic protein family in Pisum sativum L. (1993)
    Springer
    Planta 191 (1993), S. 7-17 
    Details
    ISSN: 1432-2048
    Keywords: Abscisic acid ; Developmental regulation ; Pathogenesis-related protein ; Pisum ; Protein (abscisic acid-responsive) ; Stress-inducible protein ; Tissue-specific protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The expression of members of two closely related abscisic acid (ABA)-responsive pea protein families, ABR17 and ABR18 (ABA-responsive 17200-Mr and 18100-Mr, respectively), is developmentally, tissueand stress-specifically regulated. Two-dimensional polyacrylamide gel electrophoresis revealed a number of ABR polypeptides on fluorographs of immunoprecipitated translation products of mRNAs, depending on the tissue, stage of development or type of stress. High endogenous ABA, or added ABA, enhanced the accumulation of translatable mRNA for specific ABR members under certain conditions, but high endogenous ABA was not a pre-requisite for accumulation of translatable ABR mRNA. The accumulation of ABR polypeptides was examined by Western blot analysis of acetate-buffer-extracted proteins. In fully expanded, young unstressed leaves, the ABR17 polypeptides (ABR18 polypeptides not detectable) accumulated to markedly higher levels in the epidermis than in the mesophyll. Dehydration stress caused an increased (ABR17) and detectable (ABR18) polypeptide accumulation which occurred predominantly in the epidermis. Detached leaves were used further to characterise factors affecting ABR polypeptide accumulation. An enhanced (ABR17) and detectable (ABR18) polypeptide accumulation occurred in the presence of ABA (10−4 M) but ABR18-polypeptide accumulation required light. The accumulation of both ABR polypeptides was stimulated in the presence of metabolisable and non-metabolisable carbohydrate sources but not in water or glutamine, indicating an osmotic rather than metabolic response. This carbohydrate-stimulated accumulation was markedly enhanced by light but unaffected by 3-(3,4-dichlorophenyl)-1,1-dimethylurea, an inhibitor of photosynthesis, indicating other photoreceptive processes besides photosynthesis were involved. The function of the ABR proteins remains unknown but their accumulation in aging tissues indicates a role in senescence. The results clearly demonstrate highly complex interactions between different environmental and developmental signals leading to the expression of these stressrelated proteins. In light of these results, the induction of protein expression of the newly-termed intracellular pathogenesis-related proteins, to which the ABR proteins are closely related, is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00240890
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  • 2
    Electronic Resource
    Electronic Resource
    Proteins arising during the late stages of embryogenesis in Pisum sativum L. (1991)
    Springer
    Planta 184 (1991), S. 14-23 
    Details
    ISSN: 1432-2048
    Keywords: Abscisic acid ; Late embryogenesis abundant proteins ; Pathogenesis-related protein ; Pisum (embryogenesis) ; Protein (abscisic acid-responsive) ; Seed development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two abscisic acid (ABA)-responsive seed proteins, ABR17 and ABR18 (ABA-responsive 17000-Mr and 18000-Mr, respectively), previously found to be induced in cultured embryos of pea (Pisum sativum L.) are major components synthesised during normal seed desiccation. The ABR17 and ABR18 proteins showed different patterns of accumulation. The ABR18 protein was abundant in the testa during early seed development but in desiccating seed it was synthesised in the embryo, indicating spacial as well as temporal regulation of expression. The ABR18 protein was undetectable soon after germination but reappeared after adding ABA. The ABR17 protein was not detected in the testa but appeared in the embryo just prior to maximum fresh weight. The ABR17 protein continued to be synthesised during germination and was also present in non-stressed leaves. A high level of endogenous ABA or added ABA increased levels of translatable ABR17 mRNA. The ABR17 and ABR18 proteins were further characterised so as to help determine their structure and function. Neither protein appeared to contain a signal peptide but both proteins appeared to be glycosylated. The proteins had similar amino-acid compositions and limited Nterminal analysis showed 56% sequence identity. Neither protein had any significant N-terminal sequence homology to any of the late embryogenesis-abundant (LEA) proteins or dehydrins. Both proteins, however, show striking homology with a pea disease-resistance-response protein and the major birch pollen allergen, indicating that the ABR17 and ABR18 proteins may be members of a distinct group of stress-induced proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00208230
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  • 3
    Electronic Resource
    Electronic Resource
    Purification and partial characterisation of two abscisic-acid-responsive proteins induced in cultured embryos ofPisum sativum L. (1989)
    Springer
    Planta 180 (1989), S. 16-23 
    Details
    ISSN: 1432-2048
    Keywords: Abscisic acid (induced proteins) ; Embryo culture ; Pisum (induced proteins) ; Protein (ABA-induced) ; Seed development ; Seed protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract When pea (Pisum sativum L.) embryos were cultured on low osmotica, with or without added abscisic acid (ABA), there was very little change in the total mRNA translation products resolved by one-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The only marked alteration was an increase in production of two low-molecular-weight proteins. The purification and partial characterisation of these two ABA-responsive seed proteins (ABR17 and ABR18) is described. Both proteins were purified to homoeneity, as judged by SDS-PAGE, from embryos cultured in the presence of ABA. Antisera were raised against both proteins. Each serum cross-reacted with the other protein, indicating that the proteins are closely related. Their apparent molecular masses (Mrs) were estimated to be 17200 (ABR17) and 18100 (ABR18) by SDS-PAGE, and 26000 by gel filtration. Both proteins were heterogeneous on isoelectric focusing. Neither protein was detected (by immunoblotting or immunoprecipitation of cell-free translation products) in embryos grown in vivo at early to mid-development stages but both were present in embryos late in development. These proteins appear to be produced late in seed development but are capable of being induced early in development by culturing embryos in vitro and are markedly enhanced by ABA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02411405
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  • 4
    Electronic Resource
    Electronic Resource
    Alterations in mitochondria associated with cytoplasmic and nuclear genes concerned with male sterility in maize (1975)
    Springer
    Theoretical and applied genetics 45 (1975), S. 315-321 
    Details
    ISSN: 1432-2242
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mitochondria isolated from etiolated shoots of a range of maize genotypes with the “Texas” cytoplasm conferring cytoplasmically-inherited male sterility, are sensitive to a pathotoxin isolated from Helmintho-sporium maydis, race T. The pathotoxin inhibits oxidation of α ketoglutarate and malate and stimulates NADH oxidation. The time taken for the pathotoxin to induce these changes is a measure of the sensitivity of the mitochondria to the pathotoxin. A range of nine different pairs of genotypes, each pair differing principally in the presence of nuclear male fertility restorer alleles has been compared in their sensitivity to pathotoxin. In every case the line carrying the restorer alleles is more resistant to the pathotoxin. The restored genotypes can be quantitatively arranged into groups which correspond to the four different sources of the restorer genes in these lines. It is suggested that the restorer genes cause changes in mitochondria, which modify the functional aberration introduced by the cytoplasmically-inherited mutation causing sterility.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276686
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  • 5
    Electronic Resource
    Electronic Resource
    Altered mitochondrial membrane activities associated with cytoplasmically-inherited disease sensitivity in maize (1975)
    Springer
    Theoretical and applied genetics 45 (1975), S. 309-314 
    Details
    ISSN: 1432-2242
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effect of Helminthosporium maydis, race T, pathotoxin on mitochondria isolated from etiolated maize seedlings with different cytoplasmic genomes was investigated. Mitochondria isolated from plants with the Texas cytoplasm, which confers male sterility and field susceptibility to Helminthosporium maydis race T, were sensitive to the pathotoxin while mitochondria from male fertile plants with normal cytoplasm, which are resistant to race T of the fungus, were resistant to the pathotoxin. The pathotoxin induced uncoupling of oxidative phosphorylation, activation of cytochrome oxidase and succinate cytochrome c reductase, inhibited electron transport at an early site in the electron transport chain and overcame the malate and succinate inhibition of ATPase in sensitive mitochondria. All of the pathotoxin-induced abnormalities are consistent with the hypothesis that the pathotoxin has a binding site on the inner membrane of sensitive but not resistant mitochondria and this site is controlled by cytoplasmic DNA. It is concluded that a component of susceptibility of maize lines to Helminthosporium maydis, race T, is the uncoupling and inhibition of mitochondrial electron transport by race T pathotoxin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276685
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  • 6
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    Normal growth of Arabidopsis requires cytosolic invertase but not sucrose synthase (2009)
    National Academy of Sciences
    Details
    Publication Date: 2009-05-21
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
    Published by National Academy of Sciences
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  • 7
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    Purification and partial characterisation of two abscisic-acid-responsive proteins induced in cultured embryos ofPisum sativum L. (1989)
    Springer
    Details
    Publication Date: 1989-12-01
    Print ISSN: 0032-0935
    Electronic ISSN: 1432-2048
    Topics: Biology
    Published by Springer
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