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  • 1
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: In high flux (1013–15 W/cm2) laser-plasma interaction, a large part of the incoming laser energy is radiated into soft x-rays. To determine both the shape of this spectrum and the conversion efficiency, we have designed and tested an absolutely calibrated broadband soft x-ray spectrometer with a high temporal resolution (100 ps). The detector in this spectrometer is a new coaxial x-ray diode coupled with a fast single shot oscilloscope (French IN7100 with 7 GHz frequency response cutoff). Both absolute calibrations (x-ray response of diodes) and relative calibrations (filters and mirrors) have used the French synchrotron beam lines at Laboratoire pour l'utilisation du Rayonnement Electromagnétique (LURE) in Orsay. The initial version of this instrument was first successfully implemented on laser plasmas experiments at the Phébus facility in France and an improved version is now operating at the Omega laser facility in Rochester, New York. The emitted x-ray spectrum is absolutely measured in 18 broad bands from 50 eV up to 20 keV. The softer bands (〈1.5 keV) combine mirror and filter responses coupled with the coaxial diode response to improve hard x-ray rejection. Intermediate energy channels (1.5 keV〈hν〈5 keV) used only a filter and coaxial diode. For the hardest channels (〉5 keV) we replace the x-ray diode (not sufficiently sensitive) with a photoconductive detector (neutron-damaged GaAs). An equivalent instrument will be designed for the future National Ignition Facility (NIF) in the United States and the Laser Mégajoule (LMJ) in France. © 2001 American Institute of Physics.
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  • 2
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: B4C/W multilayer mirrors designed for an x-ray beamline at the MEGAJOULE facility are susceptible to x-ray damage. The OCTAL laser was implemented to generate a gold plasma with a continuous x-ray spectrum (0.1 to 1.5 keV) used to irradiate the mirror. The fluence on the sample is 0.05 J/cm2 per shot. The pulse duration is about 1 ns. The temporally integrated spectrum reflected by the mirror is analyzed by a bent crystal spectrometer and recorded on photographic film. The spectral analysis suggests that an increase of the mean period of the multilayer happens during the second shot. This expansion is estimated to be 5% with respect to the unexposed mirror. After the second shot, destruction of the multilayer coating in the central part of the sample and irreversible damages in the other part characterized by a decrease of Bragg reflectivity have been observed. © 1996 American Institute of Physics.
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  • 3
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: © 2001 American Institute of Physics.
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  • 4
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The subantarctic Brassicaceae Pringlea antiscorbutica R. Br. (Kerguelen cabbage) was used as a model to study the physiological adaptations of higher plants to the subantarctic environment. 13C-nuclear magnetic resonance permitted, in combination with biochemical methods, the identification and quantification of the major solutes in leaves, stem and roots. As characterized in many Brassicaceae, proline was a major solute in all organs of the plants, and its accumulation was mainly controlled by salt stress rather than temperature. Glucose was the major soluble sugar in the leaves, whilst sucrose and starch accumulated in stems and roots. Over a period of 1 year we found strong correlations between (i) glucose content in leaves and irradiance, and (ii) starch content in non-photosynthetic organs and air temperature. The pattern of carbohydrate accumulation indirectly indicated that photosynthetis was sustained throughout the year, even during cold days when the temperature remained near 0 °C. This is consistent with the direct gas exchange measurements showing that photosynthetic capacity is mainly influenced by irradiance and weakly by temperature. Taken together, these characteristics demonstrated that the growth and development cycle occurs without a period of dormancy.
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 20 (1997), S. 0 
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Using genetic variability existing amongst nine pea genotypes (Pisum sativum L.), the biochemical basis of sink strength in developing pea seeds was investigated. Sink strength was considered to be reflected by the rate of starch synthesis (RSS) in the embryo, and sink activity in the seed was reflected by the relative rate of starch synthesis (RRSS). These rates were compared to the activities of three enzymes of the starch biosynthetic pathway [sucrose synthase (Sus), ADP-glucose pyrophosphorylase and starch synthase] at three developmental stages during seed filling (25, 50 and 75% of the dry seed weight). Complete sets of data collected during seed filling for the nine genotypes showed that, for all enzyme activities (expressed on a protein basis), only Sus in the embryo and seed coat was linearly and significantly correlated to RRSS. The contribution of the three enzyme activities to the variability in RSS and RRSS was evaluated by multiple regression analysis for the first two developmental stages. Only Sus activity in the embryo could explain, at least in part, the significant variability observed for both the RSS and the RRSS at each developmental stage. We conclude that Sus activity is a reliable marker of sink activity in developing pea seeds.
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  • 6
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Advances in Space Research 11 (1991), S. 77-85 
    ISSN: 0273-1177
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    European journal of wildlife research 34 (1988), S. 232-241 
    ISSN: 1439-0574
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Summary To evaluate the feeding pressure exerted by roe deer(Capreolus capreolus), on vegetation, the biomass within reach of feeding animals was measured in the Chizé forest (Deux Sèvres, France) in 2 types of woodland (oak and beech), and for 2 levels of population density: a high density of 20 animals/100 hectares (inside a fenced reserve) and a low density of 5 animals/100 hectares (outside the reserve). The biomass of vegetation was sampled 3 times (spring, end of summer, winter) using the same procedure each time. In the oakwood, total biomass was greater in areas of low density population than in areas with high density. In the beechwood, there was no significant difference in total biomass according to population density. In all cases, the maximum total biomass was observed in the spring sampling. Some differences were noted in the composition of the vegetation between the area inside the game reserve and the area outside the reserve.Hedera helix was very abundant outside the reserve andRuscus aculeatus and mosses were more frequent in the reserve. The feeding impact of roe deer on vegetation determines a change in the floral equilibrium permitting an increase in the abundance of plants not eaten by roe deer and causing a decrease of plants favoured by the deer.
    Abstract: Résumé Dans le but d'évaluer l'impact du chevreuil sur la végétation, la biomasse accessible à ces animaux a été évaluée dans 2 types d'association végétale (une chênaie et une hêtraie) pour 2 niveaux de densité de population dans la forêt domaniale de Chizé (Deux Sèvres, France): forte densité (20 animaux pour 100 hectares) dans la Réserve Nationale de Chasse et faible densité (5 animaux pour 100 hectares) à l'extérieur de la Réserve. Trois séries de relevés ont été effectuées avec la même méthode (printemps, fin de l'été, hiver). Dans la chênaie, la biomasse totale est plus importante en faible densité qu'en forte densité. Ce phénomène n'est pas observé dans la hêtraie. Dans tous les cas, elle est maximale au printemps. Des differences de la composition spécifique de la végétation sont observées entre la zone en réserve et l'extérieur. Le lierre(Hedera helix) est très abondant hors de la réserve. Le fragon(Ruscus aculeatus) et les mousses sont plus développées dans la réserve. L'impact du chevreuil se traduirait par une modification de la composition de l'équilibre végétal, le développement des espèces refusées par cet animal et la raréfaction des espèces appétentes.
    Notes: Zusammenfassung Zur Beurteilung des Einflusses des Rehwildes (Capreolus capreolus) auf die Vegetation wurde die diesen Tieren zugängliche Biomasse in zwei Waldgesellschaften (Eichen- und Buchenwald) mit unterschiedlicher Populationsdichte (hohe Dichte: 20 Stück/100 ha; niedrige Dichte 5 Stück/100 ha) im Staatsforst von Chizé (Frankreich) erhoben. Drei Probeaufnahmen wurden mittels der gleichen Methode (Frühjahr, Spätsommer, Winter) durchgeführt. Die Gesamtbiomasse liegt im Eichenwald höher bei niedriger als bei starker Populationsdichte. Gleiches Phänomen kann im Buchenwald nicht festgestellt werden. In allen Fällen wird die maximale Biomasse im Frühjahr erreicht. Unterschiede in der spezifischen Vegetationszusammensetzung zwischen Gehege-und Freiflächen konnten beobachtet werden. Efeu (Hedera helix) ist außerhalb des Geheges reichlich vorhanden. Segge (Ruscus aculeatus) und Moosarten sind im Gehege stark vertreten. Der Einfluß der Rehe wird durch die Veränderung des Vegetationsgleichgewichtes, der Entwicklung von verweigerten und Seltenwerden der bevorzugten Pflanzenarten, eindeutig.
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  • 8
    ISSN: 1570-7458
    Keywords: Egg laying ; preference ; host plant ; phenological stages ; phylloplane ; sugars ; amino acids ; Ostrinia nubilalis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Cette étude porte sur l'évaluation de l'importance de la phénologie des plantes dans le choix interspécifique de la pyrale du maïs (Ostrinia nubilalis Hbn.) pour pondre. Les expériences ont été poursuivies en serre en choix binaires sur plantes entières. Le choix entre le maïs et le haricot a été donné à l'insecte en différentes combinaisons de stades phénologiques. Les résultats montrent que c'est la phénologie qui gouverne la préférence plus que l'espèce végétale. Au cours de la croissance simultanée du maïs et du haricot d'une part, du maïs et du tournesol d'autre part, l'espèce préférée par la pyrale change. A certaines associations de stades phénologiques il n'y a pas de préférence entre les deux espèces, qui reçoivent alors autant de pontes l'une que l'autre. Des substances biochimiques hydrosolubles telles que les glucides et acides aminés libres présentes en surface des feuilles (phylloplan) ont été étudiées en corrélation avec le comportement de choix de site de ponte de la pyrale. Les différences relatives des quantités de fructose entre les deux espèces composantes du choix sont hautement corrélées avec la préférence de l'insecte. L'effet de cette substance est accentué par les différences relatives des quantités de proline, glucose et saccharose.
    Notes: Abstract The oviposition preference of the European Corn Borer Ostrinia nubilalis Hbn. Lepidoptera pyralidae was studied in relation to phenological stages of different plant species (Zea mays L., Helianthus annuus L., Phaseolus vulgaris L.). The experiments were done in greenhouses in binary choices. The results show that the plant phenological stages were more important than the plant species in the insect oviposition preference. When the choice was given between two plant species throughout their growth, the preference varied in relation to the development of each species. The relative differences in fructose amounts found on the leaf surfaces (phylloplane) were highly correlated with the insect oviposition preference. Amounts of proline, glucose and sucrose may accentuate this previous effect.
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  • 9
    ISSN: 1432-2048
    Keywords: Pisum ; Seed coat ; Sucrose ; Sucrose-metabolizing enzymes ; Sucrose synthase ; Starch
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The seed coat is a maternal organ which surrounds the embryo and is involved in the control of its nutrition. This study with pea (Pisum sativum L.) was conducted to understand more fully the sucrose/starch interconversions occurring in the seed coat. The concentrations of soluble sugars, the starch content, and the activities of the sucrose-metabolizing enzymes, sucrose synthase (Sus; EC 2.4.1.13), alkaline and soluble acid invertase (EC 3.2.1.26) and sucrose-phosphate synthase (SPS; EC 2.4.1.14) were compared at four developmental stages during seed filling. Among the four enzymes, only Sus activity was very high and strongly correlated with the starch concentration in the seed coat. Sucrose synthase catalyses the cleavage of sucrose in the presence of UDP into UDP-glucose and fructose. Sucrose synthase was purified from pea seed coats in a three-step protocol, consisting of diethylaminoethyl-Sephacel chromatography, gel filtration and affinity chromatography. The enzyme was characterized at the biochemical and molecular levels. Sucrose synthase exhibits biochemical properties which allow it to function in the direction of both sucrose cleavage and synthesis. The mass-action ratio of its four substrates was close to the theoretical equilibrium constant at the four developmental stages we studied. A labelling experiment on seed coats has shown that Sus activity is reversible in vivo and can produce 37% of neosynthesized sucrose in the seed coat cells (minimum value). It is concluded that Sus could play a central role in the control of sucrose concentration in the seed coat cells in response to the demand for sucrose in the embryo during the development of the seed.
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  • 10
    ISSN: 1432-2048
    Keywords: Chlorophyll-a/b-binding protein complex ; Nitrogen assimilation ; Phosphoenolpyruvate carboxylase ; Photosynthesis ; Sucrose phosphate synthase ; Zea (photosynthesis)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Maize (Zea mays L. cv. Contessa) was grown with a nitrogen supply that was just sufficient to support maximal biomass production. The third leaves from 14-to 21-d-old plants were harvested and net photosynthesis allowed to attain steady-state rates at an irradiance of either 250 or 700 μmol·m−2·s−1. Nitrogen in the form of either KNO3, KNO2 or NH4Cl was then supplied to the leaves through the transpiration stream. In all cases the addition of the nitrogen source resulted in an approximate doubling of the total amino-acid content of the leaves within 1 h. The glutamine pool increased to ten times the level found in control leaves in the light in the absence of added nitrogen. Glutamine accounted for about 21–24% of the total amino-acid content in leaves fed with 40 mM NH4Cl. Nitrate caused a rapid, but transient inhibition of the rate of net CO2 assimilation, accompanied by an increase in the activity of phosphoenolpyruvate carboxylase and a decrease in the maximum extractable activity of sucrose-phosphate synthase. This demonstrates that the activities of phospho-enolpyruvate carboxylase and sucrose-phosphate synthase are modulated by NO 3 − in the C4 plant maize, in a similar manner to that observed in C3 plants. Nitrite or ammonium feeding resulted in decreased rates of CO2 assimilation for as long as the nitrogen source was supplied. In all cases the degree of inhibition was greatest at high irradiance and least at low irradiance, even though the total amino-acid contents of the leaves were comparable at the time when maximum inhibition of CO2 assimilation occurred. Measurements of chlorophyll-a fluorescence showed that the quantum efficiency of PSII decreased and non-radiative dissipation of excitation energy increased as CO2 assimilation was inhibited by nitrate or nitrite. These metabolites had no direct effect on thylakoid PSII-based electron transport. Ammonium ions weakly inhibited O2 evolution at high concentrations. The addition of nitrogen (KNO 3 − , KNO2 or NH4Cl) caused a significant decrease in the phosphorylation state of the light-harvesting chlorophyll-a/b-binding protein of the thylakoid membranes. We conclude that the response of photosynthetic carbon assimilation and electron transport in maize is essentially similar whether nitrogen is supplied in the form of nitrate, nitrite or ammonium, with the noteworthy exception that the nitrogen-induced inhibition of photosynthesis is transient when leaves are supplied with NO 3 − but sustained when NO 2 − or NH 4 + is provided. We suggest that the observed modulation of phosphoenolpyruvate carboxylase and sucrose-phosphate synthase is mediated by the increase in the endogenous level of glutamine. Furthermore, the transient nature of the inhibition of CO2 assimilation in the case of NO 3 − , but not NO 2 − or NH 4 + , may be due to regulation of nitrate reductase.
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