ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Orange pectinesterase was isolated by ammonium sulfate fractionation followed by ion exchange on CM-Sephadex column. Two pectinesterase peaks were observed, The same pH and temperature optima (pH 7.5, temp 60°C), almost equal Michaelis constants (0.029%, 0.031%) and energy of activation values (5,600 cal mol-1, 5,740 cal mol-1) were obtained for the two pectinesterase fractions. Both enzyme fractions were inhibited by EDTA and polygalacturonic acid and were found to cause clarification of orange juice in a pattern similar to that obtained with natural juice. It can be assumed that the two pectinesterase chromatographic entities have similar catalytic properties.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2621.1980.tb06521.x
Permalink