Publication Date:
2004-11-30
Description:
Chromatin folding determines the accessibility of DNA constituting eukaryotic genomes and consequently is profoundly important in the mechanisms of nuclear processes such as gene regulation. Nucleosome arrays compact to form a 30-nanometer chromatin fiber of hitherto disputed structure. Two competing classes of models have been proposed in which nucleosomes are either arranged linearly in a one-start higher order helix or zigzag back and forth in a two-start helix. We analyzed compacted nucleosome arrays stabilized by introduction of disulfide cross-links and show that the chromatin fiber comprises two stacks of nucleosomes in accord with the two-start model.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Dorigo, Benedetta -- Schalch, Thomas -- Kulangara, Alexandra -- Duda, Sylwia -- Schroeder, Rasmus R -- Richmond, Timothy J -- New York, N.Y. -- Science. 2004 Nov 26;306(5701):1571-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Eidgenossische Technische Hochschule (ETH) Zurich, Institute for Molecular Biology and Biophysics, ETH-Honggerberg, CH-8093 Zurich, Switzerland.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15567867" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Chromatin/*chemistry/ultrastructure
;
DNA/chemistry/metabolism
;
Electrophoresis, Polyacrylamide Gel
;
Histones/chemistry/genetics/metabolism
;
Microscopy, Electron
;
Models, Biological
;
Models, Molecular
;
Multiprotein Complexes/chemistry
;
Mutation
;
Nucleosomes/*chemistry/ultrastructure
;
Protein Folding
;
Xenopus laevis
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
Permalink
