Publication Date:
1984-09-28
Description:
Embryonic chicken brain tissue cultured in media containing 35S-labeled sulfate or 32P-labeled phosphate incorporated 35S or 32P into the neural cell adhesion molecule (N-CAM). The 35S label was located in asparagine-linked carbohydrates on both glycopeptides (molecular weights, 170,000 and 140,000) but not in the sialic acid. The 32P label was detected in phosphoamino acids in the carboxyl-terminal third of both polypeptides, but the ratio of phosphoserine to phosphothreonine differed in the two species. The sulfated saccharides and phosphoamino acids may provide additional sites for functional control of N-CAM.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Sorkin, B C -- Hoffman, S -- Edelman, G M -- Cunningham, B A -- AI 11378/AI/NIAID NIH HHS/ -- HD 16550/HD/NICHD NIH HHS/ -- New York, N.Y. -- Science. 1984 Sep 28;225(4669):1476-8.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6474186" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Antigens, Surface/analysis/*metabolism
;
Brain/*metabolism
;
Cell Adhesion Molecules
;
Chick Embryo
;
Glycopeptides/analysis
;
In Vitro Techniques
;
Phosphates/analysis/*metabolism
;
Phosphorylation
;
Phosphoserine/analysis
;
Phosphothreonine/analysis
;
Sialic Acids/analysis
;
Sulfates/analysis/*metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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