Publication Date:
2011-09-17
Description:
Eukaryotic and archaeal multisubunit RNA polymerases (Pols) are structurally related and require several similar components for transcription initiation. However, none of the Pol I factors were known to share homology with transcription factor IIB (TFIIB) or TFIIB-related proteins, key factors in the initiation mechanisms of the other Pols. Here we show that Rrn7, a subunit of the yeast Pol I core factor, and its human ortholog TAF1B are TFIIB-like factors. Although distantly related, Rrn7 shares many activities associated with TFIIB-like factors. Domain swaps between TFIIB-related factors show that Rrn7 is most closely related to the Pol III general factor Brf1. Our results point to the conservation of initiation mechanisms among multisubunit Pols and reveal a key function of yeast core factor/human SL1 in Pol I transcription.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3319074/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3319074/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Knutson, Bruce A -- Hahn, Steven -- GM053451/GM/NIGMS NIH HHS/ -- R01 GM053451/GM/NIGMS NIH HHS/ -- R01 GM053451-17/GM/NIGMS NIH HHS/ -- T32 CA009657/CA/NCI NIH HHS/ -- T32 CA009657-22/CA/NCI NIH HHS/ -- T32 CA09657/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 2011 Sep 16;333(6049):1637-40. doi: 10.1126/science.1207699.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Fred Hutchinson Cancer Research Center, Division of Basic Sciences, 1100 Fairview Avenue N, Post Office Box 19024, Mailstop A1-162, Seattle, WA 98109, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/21921198" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Amino Acid Substitution
;
Humans
;
Molecular Sequence Data
;
Pol1 Transcription Initiation Complex Proteins/*chemistry/genetics/*metabolism
;
Protein Folding
;
Protein Interaction Domains and Motifs
;
Protein Structure, Secondary
;
Protein Structure, Tertiary
;
RNA Polymerase I/*metabolism
;
Saccharomyces cerevisiae Proteins/*chemistry/genetics/*metabolism
;
Sequence Alignment
;
TATA-Box Binding Protein/metabolism
;
Transcription Factor TFIIB/chemistry/metabolism
;
Transcription Factor TFIIIB/chemistry/genetics/metabolism
;
Transcription, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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