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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 60 (1955), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 256 (1975), S. 657-659 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Different types of hormones markedly suppressed serum AFP levels in newborn rats. Among them, glucocorticoids (prednisolone 3 jag g^d""1, dexamethazone 2 jag g^d"1, hydrocortisone 15 jag g"1 d"1) all induced a dramatic decrease in blood AFP (Fig. 1) together with an increase in albumin and total ...
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  • 3
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Analytical Biochemistry 158 (1986), S. 64-67 
    ISSN: 0003-2697
    Keywords: HPLC ; S-adenosyl amino acids ; adenine nucleotides ; liver
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Nuclear Physics, Section B 340 (1990), S. 245-279 
    ISSN: 0550-3213
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 91 (1979), S. 327-331 
    ISSN: 0006-291X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The green fluorescent protein (GFP) was used as a model protein to study the recombinant protein production by the strain Methylobacterium extorquens ATCC 55366. Scale-up from shake flasks to 20 l fed-batch fermentation was achieved using methanol as a sole carbon and energy source and a completely minimal culture medium. Two different expression vectors were used to express GFP. Clone PCM-GFP containing the vector pCM110 with native promoter of the methanol dehydrogenase PmxaF produced approximately 100-fold more GFP than the clone PRK-GFP containing the vector pRK310 with the heterogeneous promoter Plac. Several fed-batch fermentations with and without selective pressure (tetracycline) were run in a 20 l stirred tank fermenter using the two different clones of M. extorquens. The methanol concentration was monitored with an on-line semiconductor gas sensor in the culture broth. It was maintained at a non-toxic level of 1.4 g l−1 with an adaptative control which regulates the methanol feed rate. The same growth profile was achieved in all fermentations. The maximum growth rate (μmax) was 0.18 h−1 with an overall yield (YX/S) of 0.3 g g−1 methanol. With this high cell density fermentation process, we obtained high levels (up to 4 g l−1) of GFP with the clone PCM-GFP. The maximum specific GFP production (YGFP/X) with this clone was 80 mg g−1 representing approximately 16% of the total cell protein. Additional feeding of pure oxygen to the fermenter permitted a longer phase of exponential growth but had no effect on the total yields of biomass and GFP. The specific GFP production of clone PCM-GFP remained unaffected in the presence or absence of selective pressure (tetracycline), within the initial 50 h of the fermentation culture. These results suggest that M. extorquens ATCC 55366 could be an interesting candidate for overexpression of recombinant proteins.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 19 (1963), S. 163-164 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Résumé Des coupes minces en provenance de tissus minéralisés enrobés dans la celloidine ou la résine acrylique, sont montées sur ruban gommé et recouvertes d'une mince couche de celloidine pour être taillées avec l'appareil qui sert à couper les films. Ces préparations sont faciles à manipuler et à classer. Elles peuvent être soumises à de multiples expositions aux rayons X.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 4 (1970), S. 66-66 
    ISSN: 1432-0827
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 6 (1970), S. 280-289 
    ISSN: 1432-0827
    Keywords: Osteocytes ; Bone ; Matrix ; Trabeculae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé L'ultrastructure des ostéocytes et de la matrice osseuse adjacente a des été étudiée au niveau maxillaires de jeunes rats, après décalcification à l'E.D.T.A. Les événements caractéristiques du cycle d'évolution de la cellule et ses effets sur la substance osseuse péricellulaire, peuvent être groupés en 3 stades: 1. La période de formation, pendant laquelle l'ostéocyte, analogue à l'ostéoblaste, montre, cependant, une diminution progressive en ergastoplasme et une réduction de l'appareil de Golgi. 2. La phase de résorption (ostéolyse ostéocytaire) caractérisée par l'apparition des lysosomes et leur activité, provoquant un élargissement de la lacune, où s'accumule du matériel fibrillaire et floconneux. 3. La dégénérescence éventuelle et la mort de la cellule. Une régénérescence cellulaire (“ostéoplasie”) n'a pas été observée.
    Abstract: Zusammenfassung Die Feinstruktur der Osteozyten und der unmittelbar angelagerten Knochenmatrix wurde an den Kiefern junger mit EDTA demineralisierten Ratten untersucht. Die Ereignisse, welche den Lebenszyklus der Zelle und ihre Wirkung auf die pericelluläre Knochensubstanz markieren, wurden in 3 Phasen eingeteilt: 1. die bildende Periode, während welcher der Osteocyt dem Osteoblasten gleicht, jedoch eine stufenweise Abnahme der Menge von endoplasmatischem Reticulum und in der Größe des Golgi-Apparates zeigt; 2. der Resorptionsbeginn (Osteozyten-Osteolyse), welcher durch eine weitere Abnahme der sekretorischen Organellen und das zackige Aussehen der perilacunären Grenze charakterisiert ist; 3. schließlich die Degeneration und der Tod der Zelle. Der Nachweis einer Regeneration („Osteoplasie”) konnte nicht erbracht werden.
    Notes: Abstract The fine structure of the osteocytes and of the immediately adjacent bone matrix has been studied in the jaws of young rats demineralized with EDTA. The events marking the life cycle of the cell and their effects on the pericellular bone substance have been grouped into 3 phases. 1. The formative period, where the osteocyte resembles an osteoblast but shows a gradual decrease in the amount of endoplasmic reticulum and in the size of the Golgi complex. 2. The beginning of resorption (osteocytic osteolysis) which is characterized by a further decrease of the secretory organelles and the jagged appearance of the perilacunar border. Later in this phase there is further development and activity of the lysosomes resulting in increased widening of the lacuna and accumulation in the lacuna of fibrillar and flocculent material. 3. The eventual degeneration and death of the cell. No evidence of regeneration (“osteoplasia”) has been observed.
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  • 10
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Carbidopa and levodopa in plasma ; Electrochemical detection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A validated reversed-phase high-performance liquid chromatographic procedure employing electrochemical detection (LCEC) for the analysis of carbidopa and levodopa in human plasma is reported. The method is sensitive and specific with amperometric detection at a glassy carbon working electrode with Eapp=0.75 V vs. Ag/AgCl. The retention times of levodopa, internal standard, and carbidopa are 3.3, 4.5, and 9.7 minutes, respectively, with an overall chromatographic run time of 12.0 minutes. The peak height ratio versus plasma concentration is linear over the range of 5.0 to 500 ng/mL for each analyte and exhibits correlation coefficients of 0.9957 or better (n=9). The mean absolute recovery of carbidopa and levodopa using the described assay is 36.6 and 66.0%, respectively. The inter- and intra-day accuracy and precision are within 11.8% of the actual values for all concentrations. Also, due to the demonstrated instability of carbidopa and levodopa in plasma a procedure is provided to circumvent this. Blood collected in pre-treated Vacutainer tubes can be stored in an ice bath for up to 4 hours without any significant degradation, thereby providing a practical means for processing several clinical samples simultaneously.
    Type of Medium: Electronic Resource
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