ISSN:
1432-119X
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract The distribution and the levels of Gi1 (plus Gi3), Gi2, and Go in rat retina were studied immunohistochemically and immunochemically during development. At embryonic day (E) 15, Gi1α/Gi3α was observed in the inner layer of the neural retina, the future nerve fiber layer (NFL), while Gi2α was observed both in the inner and outer layers of the neural retina. No immunoreactivity for Goα was observed. At E18, Gi1α/Gi3α and Gi2α appeared in the inner plexiform layer (IPL), while Goα was faintly immunoreactive only in the NFL. At birth, Gi2α/Gi3α and Goα appeared in the ganglion cell layer. Gi2α was intensely immunoreactive in the NFL and IPL. At postnatal day (P) 10, the inner portions of the retina, from the NFL to the outer plexiform layer, were immunoreactive to Gi1α/Gi3α, Gi2α, and Goα. Gi1α/Gi3α and Goα were distributed characteristically in a laminated pattern in the IPL, but Gi2α was present homogeneously in the IPL. At P12, Gi2α appeared in the outer nuclear layer. As the postnatal days advanced, the laminated pattern of immunoreactivity to Goα in the IPL became diffuse, but immunoreactivity to Gi1α/Gi3α remained. The results of enzyme immunoassays showed that the concentration of Goα increased rapidly from P10 to P15 and reached almost the adult level at P20–P30, while Gi2α decreased until P15 and was almost constant thereafter. These results showed that the distribution of Gi1α/Gi3α, Gi2α, and Goα differs during development, suggesting that each G protein in the developing retina has a unique function.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004180050037
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