ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
Active cytosolic (CT) Cu,Zn superoxide dismutase from Schistosoma mansoni (SmCTSOD) was recovered after thrombin cleavage of a glutathione-S-transferase linked fusion protein (GST-SmCTSOD) expressed in the presence of the active-site metals. Crystals have been obtained in two space groups, P212121 and P21. The former have unit-cell parameters a = 74.64, b = 78.24, c = 95.18 Å and typically diffract to 2.2 Å. The monoclinic crystals have unit-cell parameters a = 39.27, b = 95.08, c = 78.41 Å, β = 103.55° and diffract to at least 1.55 Å. The calculated solvent content of the crystals is compatible with two dimers of SmCTSOD in the asymmetric unit in both cases. Molecular-replacement solutions have been obtained for both crystal forms and show that slight distortions in the crystal packing relate one form to the other.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S0907444901014500
Permalink