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  • 1
    Electronic Resource
    Electronic Resource
    CDK-dependent phosphorylation of Sld2 and Sld3 initiates DNA replication in budding yeast (2007)
    [s.l.] : Nature Publishing Group
    Nature 445 (2007), S. 328-332 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] In eukaryotic cells, cyclin-dependent kinases (CDKs) have an important involvement at various points in the cell cycle. At the onset of S phase, active CDK is essential for chromosomal DNA replication, although its precise role is unknown. In budding yeast (Saccharomyces cerevisiae), the ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nature05465
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  • 2
    Electronic Resource
    Electronic Resource
    S-Cdk-dependent phosphorylation of Sld2 essential for chromosomal DNA replication in budding yeast (2002)
    [s.l.] : Macmillian Magazines Ltd.
    Nature 415 (2002), S. 651-655 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Cyclin-dependent protein kinases (Cdks) in eukaryotic cells work as a key enzyme at various points in the cell cycle. At the onset of S phase, active S-phase Cdks (S-Cdks) are essential for chromosomal DNA replication. Although several replication proteins are phosphorylated in a Cdk-dependent ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nature713
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  • 3
    Electronic Resource
    Electronic Resource
    A T7 amber mutant defective in DNA-Binding protein (1981)
    Springer
    Molecular genetics and genomics 183 (1981), S. 66-73 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A T7 amber mutant, UP-2, in the gene for T7 DNA-binding protein was isolated from mutants that could not grow on sup + ssb-1 bacteria but could grow on glnU ssb-1 and sup + ssb +bacteria. The mutant phage synthesized a smaller amber polypeptide (28,000 daltons) than T7 wild-type DNA-dinding protein (32,000 daltons). DNA synthesis of the UP-2 mutant in sup + ssb-1 cells was severely inhibited and the first round of replication was found to be repressed. The abilities for genetic recombination and DNA repair were also low even in permissive hosts compared with those of wild-type phage. Moreover, recombination intermediate T7 DNA molecules were not formed in UP-2 infected nonpermissive cells. The gene that codes for DNA-binding protein is referred to as gene 2.5 since the mutation was mapped between gene 2 and gene 3.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00270140
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  • 4
    Electronic Resource
    Electronic Resource
    A cis-acting locus for the stable propagation of yeast plasmid pSR1 (1987)
    Springer
    Molecular genetics and genomics 207 (1987), S. 355-360 
    Details
    ISSN: 1617-4623
    Keywords: pSR1 plasmid ; Stability control ; cis-acting locus ; Z locus ; Zygosaccharomyces rouxii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A DNA plasmid resembling 2 μm DNA of Saccharomyces cerevisiae, pSR1, isolated from a strain of Zygosaccharomyces rouxii, has a cis-acting region, Z, for plasmid stability. The Z region was delimited to a sequence of at most 383 bp in a small unique region of the plasmid. The Z region is high in A:T pairs and contains three different pairs of short (ca. 25 bp) inverted repeats with 65% to 79% homology and three copies of direct repeats of 24 to 27 bp in length with 67% to 72% homology, but does not encode a noteworthy open reading frame. It was suggested that the Z region interacts with the S product(s) encoded by the same plasmid and with a specific host factor, but not with the other stabilization factor encoded by the P locus on the sPR1 molecule.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331601
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  • 5
    Electronic Resource
    Electronic Resource
    Factors encoded by and affecting the holding stability of yeast plasmid pSR1 (1987)
    Springer
    Molecular genetics and genomics 206 (1987), S. 88-94 
    Details
    ISSN: 1617-4623
    Keywords: pSR1 plasmid ; Stability control ; Functional region ; Zygosaccharomyces rouxii ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A 2 μm DNA-like plasmid, pSR1, isolated from a strain of Zygosaccharomyces rouxii has three coding frames, P, S and R. Insertional inactivation of R completely abolished the intramolecular recombination, and the defect was complemented by an intact R frame on a coexistent plasmid molecule. The P and S regions were also transactive and important, but not essential, for the stable maintenance of the plasmid molecules. Insertional disruption of the P frame suggested that it produces a protein factor. Similar insertional disruption of the S frame affected the plasmid stability in Z. rouxii and Saccharomyces cerevisiae hosts differently, depending on whether the inserted DNA fragment was a short 8 bp SalI linker or a long (2.2 kb) DNA fragment. Results strongly suggested that the S region encodes two factors, one RNA and the other a protein, and that the S protein is compatible with a sprecific hostfactor in Z. rouxii, but not in S. cerevisiae. In addition, a cis-acting locus, Z, was found at a site in the plasmid molecule where no distinct open reading frames were located. No long direct repeats or inverted repeats were observed in the Z region, such as are found in the REP3 locus of 2 μm DNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00326541
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  • 6
    Electronic Resource
    Electronic Resource
    Molecular structure of the replication origin of a Bacillus amyloliquefaciens plasmid pFTB14 (1987)
    Springer
    Molecular genetics and genomics 210 (1987), S. 92-100 
    Details
    ISSN: 1617-4623
    Keywords: Plasmid ; Replication protein ; Replication origin ; Bacillus subtilis host ; Bacillus amyloliquefaciens plasmid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The structure of a 1.5-kb DNA sequence that is necessary and sufficient for the replication of an 8.2-kb cryptic plasmid, pFTB14, isolated from a strain of Bacillus amyloliquefaciens has been characterized. The 1.5-kb DNA sequence contains an open reading frame, rep, stretching for 1017 bp, a promoter region for rep expression, and a possible replication origin for the plasmid upstream of the promoter. The rep product is trans-active and essential for plasmid replication. The predicted rep protein is a basic protein, as are the RepC protein of pT181, RepB of pUB110 and protein A of pC194 (all these found in staphylococci) and the π protein of the R6K plasmid of Escherichia coli. The predicted rep protein has highly homologous amino acid sequences with protein A of pC194 and RepC of pUB110 throughout the protein molecule, but not with RepC of pT181, π of R6K or protein RepH encoded by and iniating the replication of pC194.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00337763
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  • 7
    Electronic Resource
    Electronic Resource
    The CDC26 gene of Saccharomyces cerevisiae is required for cell growth only at high temperature (1992)
    Springer
    Molecular genetics and genomics 231 (1992), S. 329-331 
    Details
    ISSN: 1617-4623
    Keywords: Saccharomyces cerevisiae ; Cell division cycle ; CDC26 ; Nuclear division
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have cloned and sequenced the wild-type CDC26 gene and a mutant allele, cdc26-1, of Saccharomyces cerevisiae. Nucleotide sequence analysis revealed that the gene we cloned was the same as SCD26, a dosage-dependent suppressor of cdc26. However, the cloned gene is in fact the CDC26 gene, because a nucleotide substitution in cdc26-1 was found to be a nonsense mutation in this sequence. Disruption of this gene conferred thermosensitive cell growth and the disrupted cdc26 gene could not complement the cdc26-1 mutant allele. Thus, the CDC26 gene is required for cell growth only at high temperature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00279807
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  • 8
    Electronic Resource
    Electronic Resource
    A gene, SMP2, involved in plasmid maintenance and respiration in Saccharomyces cerevisiae encodes a highly charged protein (1993)
    Springer
    Molecular genetics and genomics 236 (1993), S. 283-288 
    Details
    ISSN: 1617-4623
    Keywords: Plasmid stability ; pSR1 plasmid ; rho mutation ; smp mutation ; Yeast plasmid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The smp2 mutant of Saccharomyces cerevisiae shows increased stability of the heterologous plasmid pSR1 and YRp plasmids. A DNA fragment bearing the SMP2 gene was cloned by its ability to complement the slow growth of the smp2 smp3 double mutant (smp3 is another mutation conferring increased stability of plasmid pSR1). The nucleotide sequence of SMP2 indicated that it encodes a highly charged 95 kDa protein. Disruption of the genomic SMP2 gene resulted in a respiration-deficient phenotype, although the cells retained mitochondrial DNA, and showed increased stability of pSR1 like the original smp2 mutant. The fact that the smp2 mutant is not always respiration deficient and shows increased pSR1 stability even in a rho 0 strain lacking mitochondrial DNA suggested that the function of the Smp2 protein in plasmid maintenance is independent of respiration. The SMP2 locus was mapped at a site 71 cM from lys7 and 21 cM from ilv2/SMR1 on the right arm of chromosome XIII.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00277124
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  • 9
    Electronic Resource
    Electronic Resource
    A specific host factor binds at a cis-acting transcriptionally silent locus required for stability control of yeast plasmid pSR1 (1993)
    Springer
    Molecular genetics and genomics 238 (1993), S. 120-128 
    Details
    ISSN: 1617-4623
    Keywords: Cis-acting locus ; Plasmid stability ; pSR1 plasmid ; Yeast plasmid ; Z locus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cis-acting locus, Z, of plasmid pSRl functions in stable maintenance of the plasmid in the native host, Zygosaccharomyces rouxii. The Z locus was shown to be located in a 482 by sequence in the 5′ upstream region of an open reading frame, P, by subcloning various DNA fragments in a plasmid replicating via the ARS1 sequence of the Saccharomyces cerevisiae chromosome. Northern analysis revealed that the Z region is not transcribed in either the native host Z. rouxii or the heterologous host S. cerevisiae. The Z region is protected from microccocal nuclease attack in Z. rouxii but not in S. cerevisiae, its protection depending on the product of the S gene encoded by pSR1. Gel retardation assays suggested that a factor present in nuclear extracts of Z. rouxii cells, irrespective of the presence or absence of a resident pSRI plasmid, binds to a 111 by Rsal-Sacll sequence in the Z region. These findings suggest that a host protein binds to the Z locus and that the S product interacts with this DNA-protein complex and stabilizes pSRl.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00279538
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  • 10
    Electronic Resource
    Electronic Resource
    Mutations in a Saccharomyces cerevisme host showing increased holding stability of the heterologous plasmid pSRI (1991)
    Springer
    Molecular genetics and genomics 225 (1991), S. 257-265 
    Details
    ISSN: 1617-4623
    Keywords: Plasmid stability ; pSRI plasmid ; rho mutation ; smp mutation ; Stable host
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have isolated Saccharomyces cerevisiae mutants, smp, showing stable maintenance of plasmid pSRI, a Zygosaccharomyces rouxii plasmid. The smp mutants were recessive and were classified into at least three different complementation groups. The three mutants also showed increased stability of YRp plasmids and the mutations are additive for plasmid stability. One mutation, smp1, confers a respiration-deficient (rho 0) phenotype and several Rho− mutants independently isolated by ethidium bromide treatment of the same yeast strain also showed increased stabilities of pSR1 and YRp plasmids. The wild-type S. cerevisiae cells showed a strongly biased distribution of pSR1 molecules as well as YRp plasmids to the mother cells at mitosis, while the smpf mutant did not show this bias. Another mutation, smp3, at a locus linked to ade2 on chromosome XV, confers temperature-sensitive growth. The SMP3 gene encodes a 59.9 kDa hydrophobic protein and disruption of the gene is lethal.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269857
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