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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 784 (1996), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1546-1718
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] Defects in the conversion of androstenedione to testosterone in the fetal testes by the enzyme 17β–hydroxysteroid dehydrogenase (17β–HSD) give rise to genetic males with female external genitalia. We have used expression cloning to isolate cDNAs encoding a microsomal ...
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  • 3
    ISSN: 1432-1432
    Keywords: Drosophila ; dec-1 locus ; Restriction map conservation ; Sequence comparison ; Melanogaster species subgroup ; Phylogeny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have analyzed ∼18 kb of DNA in and upstream of thedefective chorion-1 (dec-1) locus of the eight known species of themelanogaster species subgroup ofDrosophila. The restriction maps ofD. simulans, D. mauritiana, D. sechellia, D. erecta, andD. orena are shown to have basically the restriction map ofD. melanogaster, whereas the maps ofD. teissieri andD. yakuba were more difficult to align. However, the basic amount of DNA and sequence arrangement appear to have been conserved in these species. A small deletion of varying length (65–200 bp) is found in a repeated sequence of the central transcribed region ofD. melanogaster, D. simulans, andD. erecta. Restriction site mapping indicated that thedec-1 gene is highly conserved in themelanogaster species subgroup. However, sequence comparison revealed that the amount of nucleotide and amino acid substitution in the repeated region is much larger than in the 5′ translated region. The 5′ flanking region showed noticeable restriction site polymorphisms between species. Based on calculations from the restriction maps a dendrogram was derived that supports earlier published phylogenetic relationships within themelanogaster species subgroup except that theerecta-orena pair is placed closer to themelanogaster complex than toD. teissieri andD. yakuba.
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  • 4
    ISSN: 1432-1432
    Keywords: Drosophila ; dec-1 eggshell gene ; Wild-type variants ; Repeated region ; DNA sequencing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Thedec-1 eggshell gene inDrosophila melanogaster encodes follicle cell proteins required for proper eggshell assembly. As shown by Southern and Northern analyses thedec-1 gene occurs in four alleles (Fcl-4) among wild-type strains. Its second exon has a distinct feature in the form of 12 repeats with 78–91 nucleotides; the first five show nearly 100% homology. DNA sequence comparison of the repeated region of the alleles revealed that the length polymorphisms are caused by changes in the numbers of the first five repeats. The results suggest that the alleles have been generated by unequal intragenic crossing-over and/or slippage during DNA replication and that the allelic length variants have arisen independently. The possiblilty that the most common allele,FC1, has a selective advantage over the other alleles is discussed.
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  • 5
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 354 (1991), S. 159-161 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] To isolate cDNAs encoding additional 5a-reductases, a size-fractionated and oriented cDNA library was constructed from human prostate poly(A)+ messenger RNA in a pCMV expression vector5. The size of cDNA pools that could be screened for expression of 5o:-reductase in cultured 293 ...
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Oecologia 76 (1988), S. 125-130 
    ISSN: 1432-1939
    Keywords: Anchusa ; pollination efficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Bumblebees foraging on the self-incompatible Anchusa officinalis fly between near neighbour plants and between near neighbour inflorescences within plants. Although many-flowered plants attracted most bumblebees these plants received fewer visits on a per flower basis than smaller plants, and each bumblebee visited a smaller proportion of the flowers. The calculated effective visitation rate per flower was highest on plants of an intermediate size. If pollen-carryover was assumed to be limited the most efficient plant was predicted to be smaller since the proportion of fertilized flowers per bumblebee visit is expected to decrease further on the largest plants in relation to the total flower number. These predictions were tested by measuring fruit-set in the field. The percentage fruit-set decreased with plant size at all sizes that were investigated. That the most efficient plant was small indicates that pollen-carryover was indeed limited. However, the low percentage fruit-set associated with large size did not present a serious problem since the total estimated seed production per plant still increased with size. Selection favoring smaller plants may be low or absent in Anchusa.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Oecologia 80 (1989), S. 540-545 
    ISSN: 1432-1939
    Keywords: Crepis tectorum ; Life-history ; r-selection ; K-selection ; Stress adaptation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Populations of the monocarpic plant Crepis tectorum were grown in a series of uniform environments to test the hypothesis that weedy populations are more r-selected than populations from a more natural habitat. Weedy populations exhibited a combination of r- and K-selected traits. The relatively rapid growth, the potential for a summer annual habit, and the relatively high fecundity that characterized at least one of the two weed populations studied were considered as r-selected traits favored in habitats of unpredictable duration. However, high levels of competition from other weedy species or from the crop in arable fields may explain at least some presumably K-selected traits observed in the weedy populations, e.g. relatively large seeds and late flowering in the summer. Results indicated that stress due to abiotic factors (strong winds, desiccation and nutrient deficiency) has been a more important selective factor than r- or K-selection, in non-weedy populations from calcareous grasslands (“alvars”) on the Baltic islands.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Water, air & soil pollution 54 (1990), S. 351-364 
    ISSN: 1573-2932
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract A temporary decline in tree growth has often been observed after liming in coniferous forests poor in N but seldom in forests rich in N. To test the hypothesis that the decline was caused by decreases in N supply, C and N mineralization were estimated in incubated soil: (1) after liming in the laboratory, and (2) after earlier liming in the field. Liming increased the C mineralization rate in needle litter, nor humus and 0 to 5 cm mineral soil for a period of 40 to 100 days at 15°C. After that period, liming had no effect on the CO2 evolution rate in materials poor in N (C:N ratios 30 to 62) but increased the CO2 evolution rate in materials rich in N (C:N ratios 24 to 28). When liming induced nitrification, the CO2 evolution rate was reduced. Liming resulted in lower net N mineralization rate in needle litter and mor humus. The reduction was more pronounced when NH4 + was the only inorganic form than when NO3 − was the predominant form. The reason is probably that chemical fixation of NH3 and amino compounds increases with increasing pH. Because of the fixation, the incubation technique most likely underestimated the mineralized N available to the roots. Taking this underestimation into consideration, liming initially reduced the N release in the litter layer. In the other soil layers, liming increased the N release in soils rich in N and had only small effects in soils poor in N. For the total N supply to the roots in the litter, humus and 0 to 5 cm mineral soil layers, liming caused a slight reduction in soils poor in N and a slight increase in soils rich in N. Data on tree growth corresponded with these results.The hypotheses that tree growth depressions can be caused by reduced N supply after liming and that tree growth increases can be caused by increased N supply after liming thus seem reasonable.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Plant systematics and evolution 184 (1993), S. 27-40 
    ISSN: 1615-6110
    Keywords: Asteraceae ; Crepis tectorum ; Geographical variation ; crossability ; reproductive isolation ; genetic analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Morphometric analyses of populations ofCrepis tectorum raised under uniform conditions support the earlier finding based on herbarium studies that extensive divergence has taken place in western Europe with a centre of diversity in the Baltic lowland area. A crossing experiment showed that subsp.pumila on the island of Öland has acquired a wide range of derived characters without the establishment of strong reproductive barriers, the only exception being a weak tendency for crosses with other populations on Öland to yield more fertile F1 offspring than crosses with weedy and non-weedy populations in adjacent regions. Canadian weed populations showed a more uniform response in crosses with subsp.pumila than did morphologically similar weed populations scattered throughout Central Europe. While F2 data suggest a monogenic basis of differences in the extent of leaf dissection, other distinctive traits appear to be governed by multiple genetic factors with individually slight effects.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Plant systematics and evolution 168 (1989), S. 19-38 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Asteraceae ; Crepis tectorum ; Phenotypic plasticity ; morphometry ; norm of reaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two experiments were carried out using two different approaches to compare populations ofCrepis tectorum (Asteraceae). One was based on a comparison of means of various vegetative and reproductive characters and another was based on a comparison of response patterns of the same characters in a series of environments. Population divergence within two earlier recognized form series, one from weed habitats and one from alvar habitats on Baltic islands, resulted in a partially overlapping pattern in cluster analyses based on character means. However, the pattern revealed by a comparison of the direction and amount of plastic response suggested that populations within the two form series had more similar response patterns than other combinations of populations. It was concluded that patterns of plasticity may provide useful additional information on the overall similarity among taxa. An hypothesis that plants in weed populations should exhibit a greater phenotypic response to the environments than plants in alvar populations was rejected.
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