ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
A tetrathiafulvalene (TTF)-mediated, amperometric biosensor was developed for the specific determination of L-lactate. L-lactate oxidase was immobilized by adsorption and retained on the surface of a carbon-paste electrode using an electro-polymerized film of 1,3-phenylenediamine-resorcinol. The response of the biosensor was maximum at pH 7.0 and 30°C. Response was linear up to 0.6 mM lactate and had a minimum detection limit of 56 PM. Response time was less than 90 sec. When stored at 4°C response was stable for 〉 36 days and subsequently decreased. The sensor was unresponsive after 96 days, This enzyme-based biosensor was suitable for determining lactate concentrations in milk and yogurt.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2621.1995.tb05610.x
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