Publication Date:
2019
Description:
〈p〉The gut microbiota produce hundreds of molecules that are present at high concentrations in the host circulation. Unraveling the contribution of each molecule to host biology remains difficult. We developed a system for constructing clean deletions in 〈i〉Clostridium〈/i〉 spp., the source of many molecules from the gut microbiome. By applying this method to the model commensal organism 〈i〉Clostridium sporogenes〈/i〉, we knocked out genes for 10 〈i〉C. sporogenes〈/i〉–derived molecules that accumulate in host tissues. In mice colonized by a 〈i〉C. sporogenes〈/i〉 for which the production of branched short-chain fatty acids was knocked out, we discovered that these microbial products have immunoglobulin A–modulatory activity.〈/p〉
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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