ISSN:
1435-1536
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
,
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Description / Table of Contents:
Zusammenfassung An Liposomen aus Phosphatidylcholin (PC) und aus Phosphatidyläthanolamin (PE) wurde die Fluoreszenz nach Zugabe von 1-Anilin-8-Naphthalinsulfonat (ANS) und die elektrophoretische Beweglichkeit in Gegenwart verschiedener Salze gemessen. Zwischen der Intensität der Fluoreszenz und dem Zetapotential der Liposomen besteht eine enge Korrelation. Die Bedeutung des Oberflächenpotentials für die Bindung von ANS an die Liposomen wird besonders betont.
Notes:
Summary The fluorescence of 1-anilinonaphthalene-8-sulfonate (ANS), added to liposomes made of phosphatidylcholine (PC) and of phosphatidylethanolamine (PE), and the electrophoretic mobility of the liposomes were measured under the presence of various species of salts. It was found that the intensity of fluorescence was closely correlated with the zeta-potential of the liposomes. The variation of fluorescence intensity caused by the change of ionic strength in medium was represented quantitatively in terms of the Langmuir adsorption isotherm, where the electrostatic interaction acting between ANS molecule and liposome surface was properly taken into account. The significance of the surface potential on the binding of ANS to liposomes was stressed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01746688
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