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  • 1
    Electronic Resource
    Electronic Resource
    Morphogenetic potential of foliar explants in Duboisia myoporoides R.Br. (Solanaceae) (1986)
    Springer
    Plant cell reports 5 (1986), S. 27-30 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effects of serial combinations of either indole-3-acetic acid, indole-3-butyric acid or α-naphthaleneacetic acid (0.5–10.0 mg/l) with either kinetin, 6-benzyl-amino-purine, zeatin or 6-methylaminopurine (0.5–5.0 mg/l) have been investigated to assess the morphogenetic potential of foliar explants of Duboisia myoporoides. Shoot buds developed either directly or via a callus interphase. Combinations involving indole-3-acetic acid with any of the cytokinins were more effective in inducing shoot bud formation compared to those containing indole-3-butyric acid or α-napthalenacetic acid as an auxin. Among cytokinins, zeatin, kinetin and 6-benzylamino-purine were equally effective for shoot formation. However, optimum response with zeatin could be achieved at low concentrations (0.5–2.0 mg/l), while kinetin and 6-benzylamino-purine exhibited comparable efficacy at higher levels (3.0–5.0 mg/l). 6-Methylaminopurine proved least effective in all concentrations and combinations tested. Rooting of the differentiated shoots was readily achieved with α-naphthaleneacetic acid alone (0.5 mg/l) after changing the physical form of the medium from gel to static liquid. Regenerated plantlets were transferred to pots and grown to maturity in the field with a high rate of survival (80–90%).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269711
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  • 2
    Electronic Resource
    Electronic Resource
    Propagation of Indian Rhubarh (Rheum emodi Wall.) using shoot-tip and leaf explant culture (1989)
    Springer
    Plant cell reports 8 (1989), S. 493-496 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Shoot-tip explants of Rheum emodi Wall. (Polygonaceae) gave rise to multiple shoots when cultured on a Murashige and Skoog (1962) medium (MS) with 2.0 mg/l 6-benzylaminopurine (BAP) and 1.0 mg/l indole-3-butyric acid (IBA). Also, shoot buds developed from leaf explants using MS medium with 2.0 mg/l BAP and 0.25 to 1.0 mg/l indole-3-acetic acid (IAA) or IBA. Roots were induced when the resulting shoots were placed on MS medium with 1.0 mg/l IBA. Both regeneration procedures gave rise to healthy plantlets that were established in soil under glasshouse conditions at 80% frequency after hardening phase of two weeks. Regenerated plants showed a constant chromosome number of 2n=2x=22, same as the parent plant. The use of liquid shake cultures minimized the time and culture medium requirements for propagation. This procedure can be applied for the conservation and utilization of elite clones of R. emodi.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269057
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  • 3
    Electronic Resource
    Electronic Resource
    Clonal propagation of Picrorhiza kurroa royle ex benth. by shoot tip culture (1988)
    Springer
    Plant cell reports 7 (1988), S. 202-205 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A procedure has been developed for the clonal propagation of Picrorhiza kurroa Royle ex Benth. through shoot tip culture. Murashige and Skoog's medium (1962) supplemented with kinetin (3.0 to 5.0 mg/l) supported rapid proliferation of multiple shoots from the explants. Addition of indole-3-acetic acid (1.0 mg/l) to the kinetin containing medium showed marked improvement in the growth of regenerated shoots. However, presence of IAA in the medium did not alter the frequency of shoot multiplication. Rooting was readily achieved upon transferring shoots onto MS medium containing ∝-naphthaleneacetic acid (1.0 mg/l). Plantlets were successfully transferred to soil.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269324
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  • 4
    Electronic Resource
    Electronic Resource
    Establishment and multiplication of colchi-autotetraploids of Rauvolfia serpentina L. Benth. ex Kurz. through tissue culture (1987)
    Springer
    Plant cell, tissue and organ culture 10 (1987), S. 129-134 
    Details
    ISSN: 1573-5044
    Keywords: Rauvolfia serpentina ; colchi-autotetraploidy ; multiplication ; in vitro
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A tissue culture procedure was developed for the establishment and propagation of a colchi-autotetraploid of Rauvolfia serpentina for possible commercial exploitation. Multiplication of autotetraploid shoots was obtained either through axillary bud elongation on Murashige and Skoog [1] medium (MS) containing 2.65 μM (0.5 mgl−1) α-naphthaleneacetic acid and 0.33 μM (0.05 mgl−1) kinetin, or via multiple shoot formation on MS medium supplemented with 4.44 μM (1.0 mgl−1) 6-benzylaminopurine and 0.53 μM (0.1 mgl−1) α-naphthaleneacetic acid. Rooting could be induced by transferring the shoots to MS medium containing 7.95 μM (1.5 mgl−1) α-naphthaleneacetic acid alone. The plantlets, thus formed, were tetraploid in nature by cytological observations of the root tips. They exhibited 80–90% success in establishment under glass house and field conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00035910
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  • 5
    Electronic Resource
    Electronic Resource
    Micropropagation of tea (Camellia sinensis (L.) O. Kuntze) using Thidiazuron (1998)
    Springer
    Plant growth regulation 26 (1998), S. 57-61 
    Details
    ISSN: 1573-5087
    Keywords: axillary shoot multiplication ; Camellia sinensis (L.) O. Kuntze ; nodal segments ; Thidiazuron ; woody plant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of thidiazuron (TDZ) on the micropropagation of Camellia sinensis (China hybrid) was compared with that of benzylaminopurine (BAP) using nodal segments from in vitro raised seedlings. Extremely low concentrations of TDZ (1pM–100nM) alone were effective in inducing shoot bud proliferation and maintaining high rates of shoot multiplication on hormone-free media. On the other hand, higher concentrations of BAP (1–10μM) and its continued presence were required to initiate and sustain shoot proliferation. While wider ranges of BAP combined favourably with auxins like NAA or IBA, only specific combinations of TDZ and NAA were effective for shoot proliferation. TDZ treated explants yielded healthy shoots, with sturdy leaves, even during the initial stages of growth, whereas, the effect of BAP was cumulative over subcultures in attaining a high proliferative rate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006019206264
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  • 6
    Electronic Resource
    Electronic Resource
    Direct rooting and hardening of tea microshoots in the field (1999)
    Springer
    Plant cell, tissue and organ culture 58 (1999), S. 111-118 
    Details
    ISSN: 1573-5044
    Keywords: Camellia sinensis (L.) O. Kuntze
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Micropropagation of tea (Camellia sinensis (L.) O. Kuntze) has been widely attempted but commercial exploitation of this method is limited by heavy losses during the hardening procedures. In the present study, optimization of time of harvesting (spring and early summer) of microshoots, shoot size, soil pH (4.0–6.4), plant growth regulator treatment (IBA; 500 mg l-1, 30 min) CO2 (9.09/10×10−5 mol l-1 to 10.22/10×10-5 mol l-1 and 20/11×10−5 mol l-1 to 80/13×10−7 mol l-1) enrichment and light (15 μ mol m-2 s-1) conditions in specially designed hardening chambers, made a significant impact on the percent of success for hardening. Following the standardized procedure, up to 71.6% root induction and 73% survival could be achieved. Successful field transfer was also accomplished.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006374526540
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  • 7
    Electronic Resource
    Electronic Resource
    Somatic embryogenesis and plant regeneration from callus cultures of Aconitum heterophyllum Wall (1993)
    Springer
    Plant cell, tissue and organ culture 32 (1993), S. 213-218 
    Details
    ISSN: 1573-5044
    Keywords: Aconitum heterophyllum ; endangered medicinal plant ; somatic embryogenesis ; regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Plants were obtained via somatic embryogenesis in callus derived from in vitro raised leaf and petiole explants of Aconitum heterophyllum Wall. Callus was induced on a Murashige-Skoog medium supplemented with either 2,4-dichlorophenoxy acetic acid (2,4-d 1 mg l-1) and kinetin (KN 0.5 mg l-1) with coconut water (CW 10% v/v) or naphthalene acetic acid (NAA 5 mg l-1) and benzylaminopurine (BAP 1 mg l-1). Somatic embryos appeared after 2–3 months or 2 subculture passages when 2,4-d or NAA induced source of the callus was transferred to a MS medium containing BAP (1 mg l-1) and NAA (0.1 mg l-1). For successful plantlet formation, the somatic embryos were transferred to a medium containing 1/4 strength MS nutrient with indole-3-butyric acid (IBA 1 mg l-1). Alternatively, the somatic embryos were dipped in a concentrated solution of IBA for 5 min and placed on a hormone free medium. Complete plantlets were formed after 4 weeks and were transferred successfully to soil.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00029845
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  • 8
    Electronic Resource
    Electronic Resource
    Assessment of liquid culture procedures for in vitro propagation of Rheum emodi (1993)
    Springer
    Plant cell, tissue and organ culture 34 (1993), S. 223-226 
    Details
    ISSN: 1573-5044
    Keywords: endangered plant ; shoot-tip explants ; liquid medium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Micropropagation of an endangered Indian medicinal plant, Rheum emodi Wall., was achieved on Murashige and Skoog's medium using different liquid culture procedures. Liquid static (submerged, semi-submerged and with filter paper bridge) and shake (80 and 120 rpm) culture procedures were assessed for their effects on growth and multiplication rates. Best results were obtained using liquid shake cultures, which resulted in 50% reduction in medium requirement, 37.5% reduction in time and 1.5–2.2 fold increase in growth and multiplication rate. Liquid culture-raised plantlets facilitated easy transplantation and 90–95% survived transfer to potting mix in glasshouse.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00036106
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  • 9
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    Alpha-tubulin (CsTUA) up-regulated during winter dormancy is a low temperature inducible gene in tea [Camellia sinensis (L.) O. Kuntze] (2011)
    Springer
    Details
    Publication Date: 2011-07-03
    Print ISSN: 0301-4851
    Electronic ISSN: 1573-4978
    Topics: Biology
    Published by Springer
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  • 10
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    Components of antioxidant system of Picrorhiza kurrooa exhibit different spatio-temporal behavior (2013)
    Springer
    Details
    Publication Date: 2013-09-22
    Print ISSN: 0301-4851
    Electronic ISSN: 1573-4978
    Topics: Biology
    Published by Springer
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