Publication Date:
2019
Description:
The aim of the study was to determine the Actinobacteria structure in cultivated (C) versus non-cultivated (NC) soils divided into three groups (autogenic, hydrogenic, lithogenic) with consideration its formation process in order to assess the Actinobacteria sensitivity to agricultural soil use and soil genesis and to identify factors affecting their abundance. Sixteen C soil samples and sixteen NC samples serving as controls were taken for the study. Next generation sequencing (NGS) of the 16S rRNA metagenomic amplicons (Ion Torrent™ technology) and Denaturing Gradient Gel Electrophoresis (DGGE) were applied for precise determination of biodiversity. Generally, greater abundance of Actinobacteria in the NC soils relative to the C soils was found. Moreover, it was indicated that the actinobacterial diversity depended on both the soil genesis and the land use; however, this effect directly depended on the particular family and genera. Two factors: redox potential (Eh) and total carbon (TC) seemed to had a significant effect on the diversity of Actinobacteria. More precisely, Actinobacteria from the NC soils displayed a greater affinity for each other and were clearly influenced by Eh, whilst those from the C soils were mostly influenced by TC.
Electronic ISSN:
2073-4395
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Economics
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