ALBERT — All Library Books, journals and Electronic Records Telegrafenberg

Hits per page

hits 1 - 3 | 3 hits

Sorting

Electronic Resource

Heterogeneous inbred populations are useful as sources of near-isogenic lines for RAPD marker localization (1994)

Haley, S. D. ; Afanador, L. K. ; Miklas, P. N. ; [et al.]

Springer

Theoretical and applied genetics 88 (1994), S. 337-342

add to mindlist on the mindlist

Details

ISSN: 1432-2242

Keywords: Phaseolus vulgaris L. ; Bean rust resistance ; Polymerase chain reaction (PCR) ; DNA markers ; Near-isogenic lines (NILs)

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract The development and use of RAPD markers for applications in crop improvement has recently generated considerable interest within the plant breeding community. One potential application of RAPDs is their use for “tagging” simply-inherited (monogenic) pest-resistance genes and enabling more efficient identification and selection of genotypes carrying specific combinations of resistance genes. In this report, we propose and describe the use of heterogeneous inbred populations as sources of near-isogenic lines (NILs) for targeting RAPD markers linked to major pest resistance genes. The development of these NILs for RAPD marker analyses involved a sequence of line and mass selection during successive generations of inbreeding. DNA bulks derived from the NILs were used to identify a RAPD marker (designated OK14620, generated by 5′-CCCGCTACAC-3′ decamer) that was tightly linked (2.23±1.33 centiMorgans) to an important rust [Uromyces appendiculatus (Pers.) Unger var. appendiculatus] resistance gene (Ur-3) in common bean (Phaseolus vulgaris L.). The efficiency of this approach was demonstrated by a low rate of false-positives identified, the tightness of the linkage identified, and the ability to detect polymorphism between genomic regions that are representative of the same gene pool of common bean. This method of deriving NILs should find application by researchers interested in utilizing marker-assisted selection for one or more major pest resistance genes. The identification of OK14620 should help to facilitate continued use of the Ur-3 resistance source and will now enable marker-assisted pyramiding of three different bean rust resistance sources (two previously tagged) to provide effective and stable resistance to this important pathogen.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00223642

Permalink

	Location	Call Number	Expected	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

Electronic Resource

Pyramiding genes for resistance to bean common mosaic virus (1995)

Kelly, J. D. ; Afanador, L. ; Haley, S. D.

Springer

Euphytica 82 (1995), S. 207-212

add to mindlist on the mindlist

Details

ISSN: 1573-5060

Keywords: Bean Common Mosaic Virus (BCMV) ; epistasis ; genetic resistance ; marker assisted selection ; Phaseolus vulgaris ; plant breeding ; Random Amplified Polymorphic DNA (RAPD)

Source: Springer Online Journal Archives 1860-2000

Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Notes: Summary Gene pyramiding in Phaseolus vulgaris is being utilized to develop more effective resistance to the temperature-insensitive-necrosis-inducing (TINI) strains of Bean Common Mosaic Virus (BCMV) present in the USA. Our data indicate that contrary to previous work, the bc-3 gene is effective against these strains in the absence of the strain unspecific bc-u gene in genotypes possessing the I gene. The epistatic bc-3 gene interferes with traditional efforts to pyramid the other recessive resistance genes by masking their activity. Indirect selection based on markers linked to the other recessive resistance genes would likewise be ineffective without the ability to also select for the bc-u gene which is required for expression of the bc-2 2 gene in germplasm carrying the I gene. Because the most resistant genotype (I, bc-u, bc-I 2, bc-22, bc-3) can only be introduced into a wide range of germplasm through the use of molecular markers linked to the different resistance genes, the search for a marker linked to the strain unspecific bc-u gene should also be given priority.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00029562

Permalink