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  • 1
    Electronic Resource
    Electronic Resource
    Enzymic polyadenylation of 5S ribosomal ribonucleic acid and synthesis of a complementary deoxyribonucleic acid (1979)
    s.l. : American Chemical Society
    Biochemistry 18 (1979), S. 3232-3242 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00582a007
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  • 2
    Electronic Resource
    Electronic Resource
    Selective in vitro transcription of the 5S RNA genes of a DNA template (1979)
    s.l. : American Chemical Society
    Biochemistry 18 (1979), S. 3243-3248 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00582a008
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  • 3
    Electronic Resource
    Electronic Resource
    A Modular Vector for Agrobacterium Mediated Transformation of Wheat (1999)
    Springer
    Plant molecular biology reporter 17 (1999), S. 323-331 
    Details
    ISSN: 1572-9818
    Keywords: Agrobacterium ; modular vector ; transformation ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Wheat (cv Chinese Spring) tissues were transformed using Agrobacterium tumefasciens and a new plasmid modular vector, pMVTBP. We constructed pMVTBP with unique restriction sites connecting (1) the CaMV 35S promoter, (2) a Kozak sequence, (3) the FLAG epitope, (4) the (His)6 epitope, (5) a coding region (for wheat TATA Binding Protein, wTBP) and (6) the CaMV 35S 3′UTR. This vector thus allows easy exchange of different regulatory or coding sequences. Explants of either germinating mature seeds, or immature embryos, were induced to callus for up to two weeks, treated with virulence-induced bacteria for one hour, then regenerated into plantlets. Transient expression of a GUS reporter gene, assayed at about one week, occurred in 10–12% of calluses. Expression of the FLAG-tagged wTBP was also detected, by immunostaining. Stable expression, by selective growth on geneticin, and by GUS expression at about six weeks, occurred in 1–2% of calluses, quite comparable to that achieved by other methods.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007686408369
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  • 4
    Electronic Resource
    Electronic Resource
    Partial purification of plant transcription factors. II. An in vitro transcription system is inefficient (1987)
    Springer
    Plant molecular biology 9 (1987), S. 159-169 
    Details
    ISSN: 1573-5028
    Keywords: in vitro transcription ; RNA polymerase II ; wheat germ ; transcription factors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Crude wheat germ nuclear extracts contain many inhibitors of transcription which need to be removed before an active system can be developed. Using ion exchange column chromatography to resolve RNA polymerase II transcription components we can identify at least four fractions required for transcription by their ability to interact with, or substitute for, particular HeLa fractions. Inhibitors can be removed by a second or third chromatographic process applied to each fraction. Two plant fractions can each effectively replace the corresponding fraction in a HeLa transcription system, and the wheat fractions can work together and replace two HeLa fractions. These plant factors chromatograph identically to HeLa factors on ion exchange columns. The third fraction does not fully substitute for the corresponding HeLa fraction, but can complement this HeLa fraction when both are added at half-optimal levels. An in vitro plant system consisting of four plant chromatographic fractions will selectively transcribe a gene, but only at very low efficiency. The apparent block to greater efficiency is in elongation of the RNA past the 20–30n size.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00015648
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  • 5
    Electronic Resource
    Electronic Resource
    Partial purification of plant transcription factors. I. Initiation (1987)
    Springer
    Plant molecular biology 9 (1987), S. 147-158 
    Details
    ISSN: 1573-5028
    Keywords: initiation factors ; RNA polymerase II ; wheat germ ; transcription
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Crude plant cell protein extracts prepared from wheat germ are inactive for in vitro transcription by RNA polymerase II. These extracts do, however, have correct initiation of transcription by RNA polymerase II. Initiation is monitored by measuring the formation of transcription complexes in vitro. A nuclear extract produces more initiation events than a whole cell extract or a cytosol extract. Some factors necessary for initiation can be separated from other proteins, including inhibitors, by ion exchange column chromatography. One specific fraction is sufficient for the formation of transcription complexes and several other fractions may be stimulatory or accessory factors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00015647
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  • 6
    Electronic Resource
    Electronic Resource
    Coactivators and TAFs of transcription activation in wheat (1997)
    Springer
    Plant molecular biology 35 (1997), S. 1037-1043 
    Details
    ISSN: 1573-5028
    Keywords: coactivators ; gene regulation ; plants ; TAFs ; transcription ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Transcription regulation often activates quiescent genes in a tissue-specific or developmental manner. Activator proteins bind to a DNA sequence upstream of the promoter, interact with the general transcription proteins via bridging proteins, and elevate transcription levels. One group of bridging proteins, the coactivators, have been characterized in animals as polypeptides tightly associated with the general transcription factor TATA-binding protein (TBP). They are referred to as TAFs (TBP-associated factors), and together with TBP comprise general transcription factor IID. We provide biochemical evidence that wheat IID contains coactivators. An activator protein with an acidic activation domain facilitates the binding of IID to the template, and potentiates activated in vitro transcription with wheat IID, but not with wheat TBP. Using antibodies to wheat TBP, we demonstrate that wheat IID also contains TAFs. This is the first demonstration that a plant contains coactivators and TAFs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005930118379
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  • 7
    Electronic Resource
    Electronic Resource
    Culture of mouse preimplantation embryos as a quality control assay for human in vitro fertilization (1984)
    New York, NY : Wiley-Blackwell
    Gamete Research 9 (1984), S. 145-152 
    Details
    ISSN: 0148-7280
    Keywords: mouse ; embryo ; in vitro fertilization ; culture ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Techniques for culturing preimplantation mouse embryos from the two-cell stage to blastocyst are described, and the importance of this system to quality control assay the media and supplements used in human in vitro fertilization (IVF) procedures is discussed. Embryos from B6CBAF1 mice were cultured in a commonly used mouse culture medium, modified Krebs-Ringer-bicarbonate (Krebs'), or in a commonly used human culture medium, Ham's F10 nutrient mixture supplemented with human fetal cord serum (FCS), and results were not significantly different. Using the mouse embryo culture system, tests on 174 preparations of FCS resulted in 24.1% producing less than 75% morula or blastocyst stages after 72 h in culture, compared to 9.5% of the Krebs' control cultures. Results of the mouse embryo culture system using 98 FCS subsequently used in human IVF were compared to results from the VIP Human In Vitro Program of Eastern Virginia Medical School of Norfolk, Virginia, from June 1982 through June 1983. The data suggest that prescreening of media and supplements using this mouse embryo culture system may indicate sources of factors potentially detrimental to the success of human IVF procedures.
    Additional Material: 4 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120090204
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  • 8
    Electronic Resource
    Electronic Resource
    Comparison of strains and culture media used for mouse in vitro fertilization (1983)
    New York, NY : Wiley-Blackwell
    Gamete Research 7 (1983), S. 103-109 
    Details
    ISSN: 0148-7280
    Keywords: mouse ; strains ; media ; fertilization ; in vitro ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Success rates of superovulation in response to gonadotropic hormone treatment and in vitro fertilization (ie, mitotic cleavage following insemination) of mouse eggs from outbred CD-1, hybrid CB6Fl, or hybrid B6CBAF1, mice were compared using either a mouse inseminationmedium, modified Krebs-Ringer-bicarbonate (m-KRB), or a human insemination medium, Ham's F10 nutrient mixture. Inseminations were performed in either organ culture dishes or screw-top, flat-side tissue culture tubes. Mean superovulation rates (± SD) were 24.2 (5.1) for CD-1, 33.0 (5.8) for CB6F1, and 16.3 (6.6) for B6CBAF1 mice. For in vitro cleavage the best combination of mouse strain, insemination medium, and culture container was achieved using CB6F1, mice, m-KRB medium, and culture tubes. However, Ham's medium used with either hybrid mouse strain was shown to be employable for fertilization of mouse eggs in vitro as a quality control assay and/or experimental model system for testing the human in vitro fertilization procedure.
    Additional Material: 4 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120070202
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  • 9
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    The impact of cloud vertical profile on liquid water path retrieval based on the bi-spectral method: A theoretical study based on large-eddy simulations of shallow marine boundary-layer clouds (2016)
    Wiley
    Details
    Publication Date: 2016-04-08
    Description: Passive optical retrievals of cloud liquid water path (LWP), like those implemented for MODIS, rely on cloud vertical profile assumptions to relate optical thickness (τ) and effective radius ( r e ) retrievals to LWP. These techniques typically assume shallow clouds are vertically homogeneous; however, an adiabatic cloud model is plausibly more realistic for shallow marine boundary layer (MBL) cloud regimes. In this study a satellite retrieval simulator is used to perform MODIS-like satellite retrievals, which in turn are compared directly to the LES output. This satellite simulator creates a framework for rigorous quantification of the impact that vertical profile features have on LWP retrievals, and it accomplishes this while also avoiding sources of bias present in previous observational studies. The cloud vertical profiles from the LES are often more complex than either of the two standard assumptions and the favored assumption was found to be sensitive to cloud regime (cumuliform/stratiform). Confirming previous studies, drizzle and cloud-top entrainment of dry air are identified as physical features that bias LWP retrievals away from adiabatic and toward homogeneous assumptions. The mean bias induced by drizzle-influenced profiles was shown to be on the order of 5-10 g/m 2 . In contrast, the influence of cloud-top entrainment was found to be smaller by about a factor of two. A theoretical framework is developed to explain variability in LWP retrievals by introducing modifications to the adiabatic r e profile. In addition to analyzing bi-spectral retrievals we also compare results with the vertical profile sensitivity of passive polarimetric retrieval techniques.
    Print ISSN: 0148-0227
    Topics: Geosciences , Physics
    Published by Wiley on behalf of American Geophysical Union (AGU).
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  • 10
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    Crystal structure of human Charcot–Leyden crystal protein, an eosinophil lysophospholipase, identifies it as a new member of the carbohydrate-binding family of galectins (1995)
    Cell Press
    Details
    Publication Date: 1995-12-01
    Print ISSN: 0969-2126
    Electronic ISSN: 1878-4186
    Topics: Biology , Medicine
    Published by Cell Press
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