Publication Date:
1992-01-24
Description:
The sporogonic cycle of the avian malaria parasite Plasmodium gallinaceum was completed in vitro. Ookinetes (motile zygotes) were seeded onto a murine basement membrane-like gel (Matrigel) in coculture with Drosophila melanogaster cells (Schneider's L2). Transformation into oocysts as well as subsequent growth and differentiation were observed in parasites attached to Matrigel and depended on the presence of L2 cells. Sporozoites were first observed on day 10 in culture. Specific circumsporozoite protein antigenicity was identified in mature oocysts and in sporozoites. It is now possible to follow the entire life cycle of Plasmodium in vitro.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Warburg, A -- Miller, L H -- New York, N.Y. -- Science. 1992 Jan 24;255(5043):448-50.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Malaria Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1734521" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Antigens, Protozoan/metabolism
;
Cells, Cultured
;
Drosophila melanogaster/cytology
;
Extracellular Matrix
;
In Vitro Techniques
;
Microscopy, Electron
;
Plasmodium/*growth & development/ultrastructure
;
*Protozoan Proteins
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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