Publication Date:
1993-08-27
Description:
During the assembly of immunoglobulin and T cell receptor variable region genes from variable (V), diversity (D), and joining (J) segments, the germline-encoded repertoire is further diversified by processes that include the template-independent addition of nucleotides (N regions) at gene segment junctions. Terminal deoxynucleotidyl transferase (TdT)-deficient lymphocytes had no N regions in their variable region genes, which shows that TdT is responsible for N region addition. In addition, certain variable region genes appeared at increased frequency in TdT-deficient thymocytes, which indicates that N region addition also influences repertoire development by alleviating sequence-specific constraints imposed on the joining of particular V, D, and J segments.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Komori, T -- Okada, A -- Stewart, V -- Alt, F W -- AI20047/AI/NIAID NIH HHS/ -- New York, N.Y. -- Science. 1993 Aug 27;261(5125):1171-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Howard Hughes Medical Institute, Children's Hospital, Boston, MA 02115.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8356451" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
B-Lymphocytes/enzymology/*immunology
;
Base Sequence
;
DNA Nucleotidylexotransferase/*metabolism
;
DNA Nucleotidyltransferases/metabolism
;
Gene Rearrangement, B-Lymphocyte
;
Gene Rearrangement, T-Lymphocyte
;
*Genes, Immunoglobulin
;
Immunoglobulin Joining Region/genetics
;
Immunoglobulin Variable Region/genetics
;
Mice
;
Molecular Sequence Data
;
Nucleotides/*metabolism
;
Receptors, Antigen, T-Cell/*genetics
;
T-Lymphocytes/enzymology/*immunology
;
VDJ Recombinases
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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