Publication Date:
2003-06-28
Description:
The sorting of sodium channels to axons and the formation of clusters are of primary importance for neuronal electrogenesis. Here, we showed that the cytoplasmic loop connecting domains II and III of the Nav1 subunit contains a determinant conferring compartmentalization in the axonal initial segment of rat hippocampal neurons. Expression of a soluble Nav1.2II-III linker protein led to the disorganization of endogenous sodium channels. The motif was sufficient to redirect a somatodendritic potassium channel to the axonal initial segment, a process involving association with ankyrin G. Thus, this motif may play a fundamental role in controlling electrical excitability during development and plasticity.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Garrido, Juan Jose -- Giraud, Pierre -- Carlier, Edmond -- Fernandes, Fanny -- Moussif, Anissa -- Fache, Marie-Pierre -- Debanne, Dominique -- Dargent, Benedicte -- New York, N.Y. -- Science. 2003 Jun 27;300(5628):2091-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institut National de la Sante et de la Recherche Medicale Unite 464, Institut Jean Roche, Universite de la Mediterranee, Faculte de Medecine Secteur-Nord, Boulevard P. Dramard, 13916 Marseille Cedex 20, France.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12829783" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Motifs
;
Amino Acid Sequence
;
Animals
;
Ankyrins/metabolism
;
Axons/*metabolism
;
Cell Membrane/metabolism
;
Delayed Rectifier Potassium Channels
;
Hippocampus/cytology
;
Humans
;
Ion Channel Gating
;
Molecular Sequence Data
;
Mutation
;
NAV1.2 Voltage-Gated Sodium Channel
;
Nerve Tissue Proteins/*chemistry/genetics/*metabolism
;
Neurons/metabolism
;
Patch-Clamp Techniques
;
Potassium Channels/metabolism
;
*Potassium Channels, Voltage-Gated
;
Protein Structure, Tertiary
;
Protein Transport
;
Rats
;
Recombinant Fusion Proteins/chemistry/metabolism
;
Sodium Channels/*chemistry/genetics/*metabolism
;
Transfection
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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