Publication Date:
2005-09-06
Description:
MicroRNAs are small RNA molecules that regulate messenger RNA (mRNA) expression. MicroRNA 122 (miR-122) is specifically expressed and highly abundant in the human liver. We show that the sequestration of miR-122 in liver cells results in marked loss of autonomously replicating hepatitis C viral RNAs. A genetic interaction between miR-122 and the 5' noncoding region of the viral genome was revealed by mutational analyses of the predicted microRNA binding site and ectopic expression of miR-122 molecules containing compensatory mutations. Studies with replication-defective RNAs suggested that miR-122 did not detectably affect mRNA translation or RNA stability. Therefore, miR-122 is likely to facilitate replication of the viral RNA, suggesting that miR-122 may present a target for antiviral intervention.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Jopling, Catherine L -- Yi, Minkyung -- Lancaster, Alissa M -- Lemon, Stanley M -- Sarnow, Peter -- AI40035/AI/NIAID NIH HHS/ -- AI47365/AI/NIAID NIH HHS/ -- AI63451/AI/NIAID NIH HHS/ -- GM069007/GM/NIGMS NIH HHS/ -- Wellcome Trust/United Kingdom -- New York, N.Y. -- Science. 2005 Sep 2;309(5740):1577-81.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Microbiology and Immunology, Stanford University, Stanford, CA 94305, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/16141076" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Base Pairing
;
Base Sequence
;
Binding Sites
;
Cell Line
;
Gene Expression Regulation
;
Hepacivirus/*genetics
;
Humans
;
Liver/*metabolism/*virology
;
Mice
;
MicroRNAs/chemistry/metabolism/*physiology
;
Molecular Sequence Data
;
Mutation
;
RNA, Messenger/chemistry/metabolism
;
RNA, Viral/chemistry/genetics/*metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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