Publication Date:
1990-11-09
Description:
Stage-specific gene regulation is important in determining cell function during development. Immature B cells expressing membrane-bound immunoglobulin M (mIgM) are sensitive to antigen-induced tolerance, whereas mature B cells are activated by antigen. Previous studies have established an association between Egr-1 gene induction and antigen receptor (mIgM)-mediated activation of mature B cells. Here it is shown that the immature B cell line WEHI-231 and tolerance-sensitive bone marrow-derived B cells do not express Egr-1. It is further shown that lack of inducible expression in these cells is due to specific methylation of the Egr-1 gene. Thus, covalent inactivation of an activation-associated gene may explain tolerance sensitivity at specific stages of B cell development.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Seyfert, V L -- McMahon, S B -- Glenn, W D -- Yellen, A J -- Sukhatme, V P -- Cao, X M -- Monroe, J G -- AI 23568/AI/NIAID NIH HHS/ -- CA 09940/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1990 Nov 9;250(4982):797-800.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia 19104-6084.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2237429" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
B-Lymphocytes/*immunology
;
Blotting, Southern
;
Cell Line
;
DNA/*metabolism
;
*Gene Expression Regulation
;
*Immune Tolerance
;
Immunoglobulin M/immunology
;
Methylation
;
Mice
;
Promoter Regions, Genetic
;
Restriction Mapping
;
Transcription, Genetic
;
Transcriptional Activation
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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