Publication Date:
2002-08-24
Description:
Polyketide synthases (PKSs) assemble the polyketide carbon backbone by sequential decarboxylative condensation of acyl coenzyme A (CoA) precursors, and the C-C bond-forming step in this process is catalyzed by the beta-ketoacyl synthase (KS) domain or subunit. Genetic and biochemical characterization of the nonactin biosynthesis gene cluster from Streptomyces griseus revealed two KSs, NonJ and NonK, that are highly homologous to known KSs but catalyze sequential condensation of the acyl CoA substrates by forming C-O rather than C-C bonds. This chemistry can be used in PKS engineering to increase the scope and diversity of polyketide biosynthesis.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kwon, Hyung-Jin -- Smith, Wyatt C -- Scharon, A Janelle -- Hwang, Sung Hee -- Kurth, Mark J -- Shen, Ben -- AI51689/AI/NIAID NIH HHS/ -- T32 GM08505/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2002 Aug 23;297(5585):1327-30.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Division of Pharmaceutical Sciences and, Department of Chemistry, University of Wisconsin, Madison, WI 53705, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12193782" target="_blank"〉PubMed〈/a〉
Keywords:
3-Oxoacyl-(Acyl-Carrier-Protein) Synthase/*chemistry/*metabolism
;
Acyl Coenzyme A/metabolism
;
Amino Acid Sequence
;
Binding Sites
;
Catalysis
;
Chromatography, High Pressure Liquid
;
Genes, Bacterial
;
Macrolides/chemistry/*metabolism
;
Molecular Sequence Data
;
Multienzyme Complexes/*chemistry/*metabolism
;
Multigene Family
;
Mutation
;
Protein Engineering
;
Protein Subunits
;
Sequence Alignment
;
Spectrometry, Mass, Electrospray Ionization
;
Streptomyces/genetics
;
Streptomyces griseus/*enzymology/genetics
;
Transformation, Bacterial
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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