Publication Date:
1998-03-07
Description:
GA-binding protein (GABP) is a transcriptional regulator composed of two structurally dissimilar subunits. The alpha subunit contains a DNA-binding domain that is a member of the ETS family, whereas the beta subunit contains a series of ankyrin repeats. The crystal structure of a ternary complex containing a GABPalpha/beta ETS domain-ankyrin repeat heterodimer bound to DNA was determined at 2. 15 angstrom resolution. The structure shows how an ETS domain protein can recruit a partner protein using both the ETS domain and a carboxyl-terminal extension and provides a view of an extensive protein-protein interface formed by a set of ankyrin repeats. The structure also reveals how the GABPalpha ETS domain binds to its core GGA DNA-recognition motif.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Batchelor, A H -- Piper, D E -- de la Brousse, F C -- McKnight, S L -- Wolberger, C -- New York, N.Y. -- Science. 1998 Feb 13;279(5353):1037-41.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biophysics and Biophysical Chemistry and the Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9461436" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Ankyrins/chemistry
;
Crystallography, X-Ray
;
DNA/*metabolism
;
DNA-Binding Proteins/*chemistry/*metabolism
;
Dimerization
;
GA-Binding Protein Transcription Factor
;
Hydrogen Bonding
;
Models, Molecular
;
Molecular Sequence Data
;
*Protein Conformation
;
Protein Structure, Secondary
;
Proto-Oncogene Proteins/chemistry/metabolism
;
Proto-Oncogene Proteins c-ets
;
Recombinant Proteins/chemistry/metabolism
;
Trans-Activators/chemistry/metabolism
;
Transcription Factors/*chemistry/*metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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