Publication Date:
2008-10-11
Description:
A long-standing goal of biology is to map the behavior of all cells during vertebrate embryogenesis. We developed digital scanned laser light sheet fluorescence microscopy and recorded nuclei localization and movement in entire wild-type and mutant zebrafish embryos over the first 24 hours of development. Multiview in vivo imaging at 1.5 billion voxels per minute provides "digital embryos," that is, comprehensive databases of cell positions, divisions, and migratory tracks. Our analysis of global cell division patterns reveals a maternally defined initial morphodynamic symmetry break, which identifies the embryonic body axis. We further derive a model of germ layer formation and show that the mesendoderm forms from one-third of the embryo's cells in a single event. Our digital embryos, with 55 million nucleus entries, are provided as a resource.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Keller, Philipp J -- Schmidt, Annette D -- Wittbrodt, Joachim -- Stelzer, Ernst H K -- New York, N.Y. -- Science. 2008 Nov 14;322(5904):1065-9. doi: 10.1126/science.1162493. Epub 2008 Oct 9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Cell Biology and Biophysics Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany. keller@embl.de〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/18845710" target="_blank"〉PubMed〈/a〉
Keywords:
Algorithms
;
Animals
;
Body Patterning
;
*Cell Division
;
Cell Nucleus/physiology
;
Databases, Factual
;
Embryo, Nonmammalian/*cytology
;
*Embryonic Development
;
Endoderm/embryology
;
Germ Layers/cytology/*embryology/physiology
;
Image Processing, Computer-Assisted
;
Mesoderm/embryology
;
Microscopy, Fluorescence/methods
;
Models, Biological
;
Motion Pictures as Topic
;
Mutation
;
Software
;
Zebrafish/*embryology/genetics
;
beta Catenin/analysis
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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