ISSN:
1573-3904
Keywords:
Acute phase response
;
Superoxide ion
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary Proteolysis of human C-reactive protein (CRP) by lysosomal enzymes derived from human neutrophils is shown to yield short peptides capable of modulating the production of superoxide ions by stimulated human neutrophils. Thus, fractionation of trichloroacetic acid-soluble digestion mixtures by HPLC yielded the following peptides: Ser-Tyr (1), Gly-Tyr (2), Phe-Glu-Val-Pro-Glu-Val-Thr (3), Trp-Asp-Phe-Val (4), Asn-Met-Trp-Asp-Phe-Val (5) and Gln-Leu-Trp-Pro (6). These peptides, corresponding to CRP sequences 18–19, 48–49 and/or 72–73, 84–90, 162–165, 160–165 and 203–206, respectively, have been synthesized and peptides 2, 3 and in particular peptide 6 were found to significantly inhibit neutrophilic function. The results suggest that CRP-derived peptides may be capable of regulating superoxide ion production by neutrophils in vivo during the acute phase response as part of a complex protective mechanism.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00122918
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