ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
The monocot mannose-specific lectin, Scilla campanulata agglutinin (SCA), from bluebell bulbs has a strong affinity for α1,3- and α1,6-linked mannosyl residues. SCA has been co-crystallized with the trisaccharide α-D-mannopyranosyl-(1→6)-α-D-mannopyranosyl-(1→3)-α-D-mannopyranoside {α-D-Man-(1→6)-[α-D-Man-(1→3)]-α-D-Man}, the core structure of biantennary N-linked oligosaccharides. Crystals of the complex were obtained by the hanging-drop vapour-diffusion technique. A complete data set to 2.5 Å resolution has been collected at 100 K, using a MAR image-plate system at a synchrotron source, from crystals which belong to the space group C2 with unit-cell dimensions a = 99.38, b = 119.86, c = 77.10 Å and β = 105.56°. Use of a CCD detector with cryo-cooled crystals improved the resolution to 2.3 Å. A molecular replacement solution, with the 2.5 Å data set, using the native SCA as a search model was obtained, with six subunits per asymmetric unit.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S0907444997019859
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