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  • 1
    Electronic Resource
    Electronic Resource
    Gas Vesicles (1975)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Plant Physiology 26 (1975), S. 427-439 
    Details
    ISSN: 0066-4294
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.pp.26.060175.002235
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  • 2
    Electronic Resource
    Electronic Resource
    The protein encoded by gvpC is a minor component of gas vesicles isolated from the cyanobacteria Anabaena flos-aquae and Microcyctis sp. (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 2 (1988), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The proteins present in gas vesicles of the cyanobacteria Anabaena flos-aquae and Microcystis sp. Were separated by SDS-polyacrylamide gel electrophoresis. Each contained a protein of Mr 22 K whose N-terminal amino acid sequences showed homology with that of the Calothrix sp. PCC 7601 gvpC gene product. The gvpC gene from A. flos-aquae was cloned and sequenced. The derived amino acid sequence for the gene product indicated a protein, GVPc, of 193 residues and Mr 21985 containing five highly conserved 33 amino acid repeats. The sequence was identical at the N-terminus to that of the Mr 22K protein present in gas vesicles and showed correspondence to seven tryptic peptides isolated from gas vesicles. This establishes that GVPc forms a second protein component of the gas vesicle, in addition to the main constituent, the 70 residue GVPa. Quantitative amino acid analysis of entire gas vesicles reveals that GVPc accounts for only 2.9% of the protein molecules and 8.2% of the mass present: this is insufficient to form the conical end caps of the gas vesicles. It is suggested that GVPc provides the hydrophilic outer surface of the gas vesicle wall; the 33 amino acid repeats may interact with the periodic structure provided by GVPa.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1988.tb00062.x
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  • 3
    Electronic Resource
    Electronic Resource
    A square bacterium (1980)
    [s.l.] : Nature Publishing Group
    Nature 283 (1980), S. 69-71 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Gas vacuoles are found exclusively in prokaryotic organisms and occur mainly in planktonic forms1'2. It has been proposed that, through providing or regulating buoyancy, they are important in positioning these organisms at particular depths in natural waters2. Surveys of freshwater lakes3'4 suggest ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/283069a0
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  • 4
    Electronic Resource
    Electronic Resource
    Isolation and Purification of Intact Gas Vesicles from a Blue–Green Alga (1969)
    [s.l.] : Nature Publishing Group
    Nature 224 (1969), S. 716-717 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Large quantities of the alga Anabaena flos-aquae were grown in carboys and concentrated as described earlier3'4. The cells were lysed by the rapid addition of sucrose solution to a final concentration of 0-7 M. It is thought that lysis results from the cell wall being placed under tension by the ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/224716a0
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  • 5
    Electronic Resource
    Electronic Resource
    Metabolic Activities of Isolated Heterocysts of the Blue-green Alga Anabaena cylindrica (1966)
    [s.l.] : Nature Publishing Group
    Nature 209 (1966), S. 94-95 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] In making cell-free extracts of A. cylindrica3 by smashing filaments in a French press4, it was noticed that some of the heterocysts resisted breakage even at pressures exceeding 2,100 atm. At lower pressures (760 atm.) it was found that most of the heterocysts remained intact while all the ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/209094a0
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  • 6
    Electronic Resource
    Electronic Resource
    Is the Heterocyst the Site of Nitrogen Fixation in Blue-green Algae? (1968)
    [s.l.] : Nature Publishing Group
    Nature 220 (1968), S. 810-812 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] In a previous communication6 two of us reported on a method of "differential cell disruption" for the isolation of heterocysts from the filaments of Anabaena cylindrica. These isolated heterocysts were tested for nitrogen fixation using the nitrogen-15 technique, but no activity was detected. We ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/220810b0
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  • 7
    Electronic Resource
    Electronic Resource
    Lipid Composition of Heterocysts (1969)
    [s.l.] : Nature Publishing Group
    Nature 221 (1969), S. 673-674 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The alga was grown in nitrogen-free medium, with shaking, using special culture flasks3. Heterocysts were isolated and purified using the differential cell disruption technique described previously4 except that the algal material collected after passage through the French press was homogenized in ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/221673a0
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  • 8
    Electronic Resource
    Electronic Resource
    In the soup (1984)
    [s.l.] : Nature Publishing Group
    Nature 312 (1984), S. 386-386 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] EACH springtime in lakes of temperate climes brings the stirring of a thin alphabet soup of phytoplankton: Asterionella, the Biddulphiales, Closterium, Dinobryon and the Euglenales.... The soup thickens as the temperature rises and the days lengthen; species a outcompetes b to form the spring bloom ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/312386b0
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  • 9
    Electronic Resource
    Electronic Resource
    A study of the strength and stability of gas vesicles isolated from a blue-green alga (1971)
    Springer
    Archives of microbiology 79 (1971), S. 327-337 
    Details
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The strength of intact gas vesicle membranes isolated from the bluegreen alga Anabaena flos-aquae was investigated under different physical and chemical conditions some of which might be important in determining their stability in living cells. High concentrations of urea and of divalent cations, pH's above 9 and below 7, exposure to proteolytic enzymes and temperatures over 40°C, all resulted in weakening of the gas vesicles, as indicated by changes in their range of critical-collapse pressure. Detergents gave no apparent increase in critical pressure, suggesting that the interfacial tension at the outer surface of the membrane is very small. Taking these properties into consideration a simple method of isolating intact gas vesicles was improved to give better than 50% recovery and membranes of 97.6% purity. The gas vesicles prepared in this way could be preserved intact by freeze-drying. The stability of gas vesicles in different environments is discussed in relation to their proteinaceous composition.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00424908
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  • 10
    Electronic Resource
    Electronic Resource
    Chemical composition of gas vesicles isolated from Anabaena flos-aquae (1972)
    Springer
    Archives of microbiology 85 (1972), S. 304-309 
    Details
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Chemical analysis of purified preparations of gas vesicles isolated from the filamentous blue-green alga Anabaena flos-aquae has shown that they are similar in composition to those isolated from the unicellular alga Microcystis aeruginosa by Jones and Jost (1970), being proteinaceous structures, free of lipid and carbohydrate. The gas vesicle protein from Anabaena contains the same 14 amino acids, in broadly the same proportions; in addition there is a small proportion of proline. No sulphur-containing amino acids are present. The empirical formula, suggested by the amino acid ratios, indicates a molecule of 15000 MW.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00549268
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